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Article
Cytokine changes in colonic mucosa associated with Blastocystis spp. subtypes 1 and 3 in diarrhoea-predominant irritable bowel syndrome
Parasitology
  • Javed Yakoob, Aga Khan University
  • Zaigham Abbas, Aga Khan University
  • Muhammad Waqas Usman, Aga Khan University
  • Aisha Sultana, Aga Khan Unviersity
  • Muhammad Islam, Aga Khan University
  • Safia Awan, Aga Khan University
  • Zubair Ahmad, Agha Khan University
  • Saeed Hamid, Aga Khan University
  • Wasim Jafri, Aga Khan University
Publication Date
6-1-2014
Document Type
Article
Abstract
We determined cytokines (e.g. interleukin-8, 10, 12 and TNF-α) expression by peripheral blood mononuclear cells (PBMCs) and in rectal mucosa in diarrhoea-predominant irritable bowel syndrome (D-IBS) with Blastocystis spp. Eighty patients with D-IBS and Blastocystis spp. infection were classified as 'cases' and 80 with D-IBS without Blastocystis spp. infection were classified as 'control'. Cases were subdivided into D-IBS and Blastocystis sp. defined type 1 (subtype-specific primer SB83) and type 3 (SB227). Stool microscopy and culture were performed. Rectal biopsies were obtained for histology and cytokines by real-time PCR for mRNA expression of cytokines. PBMCs IL-8 was similar in different groups but in type 1, IL-8mRNA was increased compared with type 3 (P = 0·001) and control (P = 0·001). In type 1, IL-10 by PBMCs had a low mean value (14·5±1·6) compared with (16·7±1·5) type 3 and (16±2·3) in controls (P
Citation Information
Javed Yakoob, Zaigham Abbas, Muhammad Waqas Usman, Aisha Sultana, et al.. "Cytokine changes in colonic mucosa associated with Blastocystis spp. subtypes 1 and 3 in diarrhoea-predominant irritable bowel syndrome" Parasitology Vol. 141 Iss. 7 (2014) p. 957 - 969
Available at: http://works.bepress.com/javedyakoob/50/