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Anti-Helicobacter pylori activity and inhibition of Helicobacter pylori-induced release of IL-8 in AGS cells by plant extracts
Journal of Medicinal Plant Research
  • Javed Yakoob, Aga Khan University
  • Zaigham Abbas, Agha Khan University
  • Rustam Khan, Aga Khan University
  • Muhammad Waqas Usman, Aga Khan University
  • Saeed Hamid
  • Safia Awan, Aga Khan University
  • Kanza Shamim, Aga Khan Univesity
  • Syed Faisal Zaidi, University of Toyama
  • Toshiro Sugiyama, University of Toyama
  • Wasim Jafri, Aga Khan University
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Plants used in popular diet were studied for anti-Helicobacter pylori activity and their effect on the expression of interleukin-8 (IL-8) from H. pylori infected gastric epithelial cells. Extracts were prepared of Allium sativum (A. sativum), Cuminum cyminum (C. cyminum),Piper nigrum (P. nigrum) and their mix in two different dilutions. AGS cell line and H. pyloristrains were used for co-culture experiments. Extracts bactericidal activity was determined by a viable colony count. ELISA (Enzyme linked immunosorbent assay) was used to determine IL-8 expression. DNA extracted from bacterial cells was used for polymerase chain reaction (PCR) of cytotoxin-associated gene (cagA) and E (cagE). Results revealedH. pylori strains sensitivity to A. sativum (5.5 mg/ml) was 57% (39/69) (p=0.06), and to 11 mg/ml was 65% (45/69) (p=0.02) compared to amoxicillin, respectively. CagE positive H. pylori, 37% (11/30) (p=0.02) were sensitive to plant mixture (23.5 mg/ml), 60% (18/30) (pC. cyminum (PP. nigrum (P=0.0046) and A. sativum(P=0.0021), respectively compared to positive and negative controls. Thus, dietary plants demonstrated an anti-H. pylori effect. They reduced IL-8 expression from the H. pyloriinfected AGS cells. Key words: Helicobacter pylori; Allium sativum, Cuminum cyminum, Piper nigrum, gastric epithelial cells, interleukin-8, ELISA, cagA, cagE.
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Creative Commons Attribution 4.0
Citation Information
Javed Yakoob, Zaigham Abbas, Rustam Khan, Muhammad Waqas Usman, et al.. "Anti-Helicobacter pylori activity and inhibition of Helicobacter pylori-induced release of IL-8 in AGS cells by plant extracts" Journal of Medicinal Plant Research Vol. 7 Iss. 15 (2013) p. 970 - 979
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