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Article
OCT2, SSX and SAGE1 reveal the phenotypic heterogeneity of spermatocytic seminoma reflecting distinct subpopulations of spermatogonia
Journal of Pathology (2011)
  • jasmine Lim, University of Malaya
Abstract
Spermatocytic seminoma (SS) is a rare testicular neoplasm that occurs predominantly in older men. In this study,
we aimed to shed light on the histogenesis of SS by investigating the developmental expression of protein
markers that identify distinct subpopulations of human spermatogonia in the normal adult testis. We analysed
the expression pattern of OCT2, SSX2-4, and SAGE1 in 36 SS cases and four intratubular SS (ISS) as well as a
series of normal testis samples throughout development. We describe for the first time two different types of SS
characterized by OCT2 or SSX2-4 immunoexpression. These findings are consistent with the mutually exclusive
antigenic profile of these markers during different stages of testicular development and in the normal adult testis.
OCT2 was expressed predominantly in Adark spermatogonia, SSX2-4 was present in Apale and B spermatogonia
and leptotene spermatocytes, whilst SAGE1 was exclusively present in a subset of post-pubertal germ cells, most
likely B spermatogonia. The presence of OCT2 and SSX2-4 in distinct subsets of germ cells implies that these
markers represent germ cells at different maturation stages. Analysis of SAGE1 and SSX2-4 in ISS showed spatial
differences suggesting ongoing maturation of germ cells during progression of SS tumourigenesis. We conclude
that the expression pattern of OCT2, SSX2-4, and SAGE1 supports the origin of SS from spermatogonia and
provides new evidence for heterogeneity of this tumour, potentially linked either to the cellular origin of SS or
to partial differentiation during tumour progression, including a hitherto unknown OCT2-positive variant of the
tumour likely derived from Adark spermatogonia.
Keywords
  • OCT2; SSX2-4; SAGE1; spermatocytic seminoma; spermatogonia
Publication Date
Spring April 12, 2011
DOI
0.1002/path.2919
Citation Information
jasmine Lim. "OCT2, SSX and SAGE1 reveal the phenotypic heterogeneity of spermatocytic seminoma reflecting distinct subpopulations of spermatogonia" Journal of Pathology (2011)
Available at: http://works.bepress.com/jasmine-lim/3/