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Extraction of DNA by Magnetic Ionic Liquids: Tunable Solvents for Rapid and Selective DNA Analysis
Analytical Chemistry (2015)
  • Kevin D. Clark, University of Toledo
  • Omprakash Nacham, University of Toledo
  • Honglian Yu, University of Toledo
  • Tianhao Li, University of Toledo
  • Melissa M. Yamsek, University of Toledo
  • Donald R. Ronning, University of Toledo
  • Jared L. Anderson, University of Toledo

DNA extraction represents a significant bottleneck in nucleic acid analysis. In this study, hydrophobic magnetic ionic liquids (MILs) were synthesized and employed as solvents for the rapid and efficient extraction of DNA from aqueous solution. The DNA-enriched microdroplets were manipulated by application of a magnetic field. The three MILs examined in this study exhibited unique DNA extraction capabilities when applied toward a variety of DNA samples and matrices. High extraction efficiencies were obtained for smaller single-stranded and double-stranded DNA using the benzyltrioctylammonium bromotrichloroferrate(III) ([(C8)3BnN+][FeCl3Br–]) MIL, while the dicationic 1,12-di(3-hexadecylbenzimidazolium)dodecane bis[(trifluoromethyl)sulfonyl]imide bromotrichloroferrate(III) ([(C16BnIM)2C122+][NTf2–, FeCl3Br–]) MIL produced higher extraction efficiencies for larger DNA molecules. The MIL-based method was also employed for the extraction of DNA from a complex matrix containing albumin, revealing a competitive extraction behavior for the trihexyl(tetradecyl)phosphonium tetrachloroferrate(III) ([P6,6,6,14+][FeCl4–]) MIL in contrast to the [(C8)3BnN+][FeCl3Br–] MIL, which resulted in significantly less coextraction of albumin. The MIL-DNA method was employed for the extraction of plasmid DNA from bacterial cell lysate. DNA of sufficient quality and quantity for polymerase chain reaction (PCR) amplification was recovered from the MIL extraction phase, demonstrating the feasibility of MIL-based DNA sample preparation prior to downstream analysis.

  • extraction,
  • ionic liquids,
  • nucleic acids,
  • bacterial cell lysate,
  • DNA sample preparation,
  • double stranded DNA,
  • magnetic ionic liquids,
  • tetrachloroferrate,
  • polymerase chain reaction
Publication Date
January 11, 2015
Publisher Statement
Reprinted (adapted) with permission from Analytical Chemistry, 87 (2015): 1552, doi: 10.1021/ac504260t. Copyright 2015 American Chemical Society.
Citation Information
Kevin D. Clark, Omprakash Nacham, Honglian Yu, Tianhao Li, et al.. "Extraction of DNA by Magnetic Ionic Liquids: Tunable Solvents for Rapid and Selective DNA Analysis" Analytical Chemistry Vol. 87 Iss. 3 (2015)
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