![](https://d3ilqtpdwi981i.cloudfront.net/IzGrFC7ERDNCjYYh433l5wGtYRc=/425x550/smart/https://bepress-attached-resources.s3.amazonaws.com/uploads/ea/ac/1e/eaac1eec-e4e0-4652-850f-8b834d48fbba/file.gif)
Article
Notch3 is involved in adipogenesis of human adipose-derived stromal/stem cells
Biochimie
(2018)
Abstract
Human adipose-derived stromal/stem cells (hASCs) have tremendous therapeutic potential and the ability to offer insight into human development and disease. Here we subject human ASCs to siRNA-mediated knockdown of Notch3 cultured under both self-renewing and adipogenic differentiation conditions. Self-renewal was monitored by assessing viability and proliferation rates through staining and alamarBlue assays, respectively. Adipogenesis was measured through Oil-Red O staining, western blot, and quantitative real-time RT-PCR that determined expression levels of multipotency and adipogenic markers over time. Notch3 was expressed in self-renewing hASCs but knockdown, as validated by qRT-PCR and western blot, showed no impact on cell viability, as measured through live-dead staining, or cell proliferation rates, as measured through alamarBlue assays. However, although Notch3 expression was observed to increase during adipogenesis, in the absence of Notch3 there was a significant increase in hASC adipogenesis as demonstrated through an increased number of lipid vesicles, and increased expression of adipogenic markers ppar-γ, adiponectin, fabp4, and plin2. Although Notch3 is only one of four Notch receptors expressed on the surface of hASCs, this receptor appears important for proper regulation of adipogenic differentiation, possibly serving as a negative regulator to prevent inappropriate adipogenesis or promote other lineage commitments of ASCs.
Keywords
- Notch signaling,
- Notch3,
- Adipose Stem Cells,
- Adipogenesis,
- Transcription
Disciplines
Publication Date
Summer July, 2018
Citation Information
Demi A Sandel, Mengcheng Liu, Ngozi Ogbonnaya and Jamie Newman. "Notch3 is involved in adipogenesis of human adipose-derived stromal/stem cells" Biochimie Vol. 150 (2018) p. 31 - 36 Available at: http://works.bepress.com/jamie-newman/2/