Article
MED31 involved in regulating self-renewal and adipogenesis of human mesenchymal stem cells
Molecular Biology Reports
(2018)
Abstract
Regulation of gene expression is critical for the maintenance of cell state and homeostasis. Aberrant regulation of genes can lead to unwanted cell proliferation or misdirected differentiation. Here we investigate the role of MED31, a highly conserved subunit of the Mediator complex, to determine the role this subunit plays in the maintenance of human mesenchymal stem cell (hMSC) state. Using siRNA-mediated knockdown of MED31 we demonstrate a decrease in self-renewal based on cell assays and monitoring of gene expression. In addition, in the absence of MED31, hMSCs also displayed a reduction in adipogenesis as evidenced by diminished lipid vesicle formation and expression of specific adipogenic markers. These data present evidence for a significant role for MED31 in maintaining adult stem cell homeostasis, thereby introducing potential novel targets for future investigation and use in better understanding stem cell behavior and adipogenesis.
Keywords
- Mediator Complex,
- Transcription,
- Adipogenesis,
- Human mesenchymal stem cells
Disciplines
Publication Date
Fall October, 2018
Citation Information
Erik P Beadle, Joseph A Straub, Bruce A Bunnell and Jamie Newman. "MED31 involved in regulating self-renewal and adipogenesis of human mesenchymal stem cells" Molecular Biology Reports Vol. 45 Iss. 5 (2018) p. 1545 - 1550 Available at: http://works.bepress.com/jamie-newman/1/