Article
Erysipelothrix rhusiopathiae: Association of Spa-type with Serotype and Role in Protective Immunity
Vaccine
Document Type
Article
Disciplines
Publication Version
Published Version
Publication Date
3-1-2010
DOI
10.1016/j.vaccine.2010.01.041
Abstract
A collection of swine, fish, and cetacean Erysipelothrix rhusiopathiae strains representing 16 serotypes was analyzed for possession of the three currently recognized surface protective antigen (spa)-types: spaA, spaB, and spaC. Polymerase chain reaction (PCR) assays and Western blotting with a SpaA-specific monoclonal antibody demonstrated that spa-type is not confined to specific serotype groups. In particular, the spa-type of strains of aquatic origin was more variable than those of terrestrial origin, and possessed the distinct ability to express more than one spa. In a cross-protection study, mice immunized with an E. rhusiopathiae serotype 2 SpaA-type strain and challenged with various E. rhusiopathiae isolates were completely protected against strains exhibiting a single homologous spa, but variably protected against strains possessing a heterologous spa or those harboring more than one spa-type.
Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
File Format
application/pdf
Citation Information
Alaina Louise Ingebritson, James A. Roth and Paul Joseph Hauer. "Erysipelothrix rhusiopathiae: Association of Spa-type with Serotype and Role in Protective Immunity" Vaccine Vol. 28 Iss. 13 (2010) p. 2490 - 2496 Available at: http://works.bepress.com/james_roth/43/
This article is from Vaccine 28, 2490, doi:10.1016/j.vaccine.2010.01.041.