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Article
Gradient Separation and Cytochemical Characterisation of Neutrophils from Kidney of Fathead Minnow (Pimephales Promelas Rafinesque, 1820)
Fish & Shellfish Immunology
  • Dušan Palić, Iowa State University
  • Claire B. Andreasen, Iowa State University
  • Dagmar E. Frank, Iowa State University
  • Bruce W. Menzel, United States Department of Agriculture
  • James A. Roth, Iowa State University
Document Type
Article
Publication Version
Published Version
Publication Date
3-1-2005
DOI
10.1016/j.fsi.2004.07.003
Abstract

Neutrophils are an important component of host defence against many bacterial, viral and fungal infections, and the evaluation of neutrophil function is valuable for assessment of health status [1]. Some literature is non-specific or contradictory about the classification of leucocyte morphology in fish, since morphology alone can vary by species [2e4]. Most studies document that fish neutrophils have very similar morphological and histochemical staining properties to mammalian neutrophils, and can be distinguished by the presence of myeloperoxidase in cytoplasmic granules [5]. Neutrophils are found in fish kidney, spleen and blood, and are commonly increased in inflammatory lesions [6]. There is evidence for phagocytic, chemotactic and bactericidal functions in fish neutrophils and an intense respiratory burst [7e9]. Techniques for obtaining relatively pure neutrophil populations for functional studies have not been previously reported for the fathead minnow (Pimephales promelas Rafinesque, 1820), and the characterisation of leucocyte morphology and cytochemical staining has not been adequately defined.

Comments

This article is from Fish & Shellfish Immunology 18 (2005): 263, doi:10.1016/j.fsi.2004.07.003.

Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
Date Available
2015-07-22
File Format
application/pdf
Citation Information
Dušan Palić, Claire B. Andreasen, Dagmar E. Frank, Bruce W. Menzel, et al.. "Gradient Separation and Cytochemical Characterisation of Neutrophils from Kidney of Fathead Minnow (Pimephales Promelas Rafinesque, 1820)" Fish & Shellfish Immunology Vol. 18 Iss. 3 (2005) p. 263 - 267
Available at: http://works.bepress.com/james_roth/1/