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Article
A subset of human rapidly self-renewing marrow stromal cells preferentially engraft in mice
Blood
  • R.H. Lee
  • S.C. Hsu
  • James R. Munoz, Nova Southeastern University
  • J.S. Jung
  • N.R. Lee
  • R. Pochampally
  • D.J. Prockop
Document Type
Article
Publication Date
1-1-2006
Disciplines
Peer Reviewed
1
Abstract

Controversies have arisen as to whether adult stem cells or progenitor cells from bone marrow can engraft into nonhematopoietic tissues in vivo. To resolve some of the controversies, we developed a highly sensitive polymerase chain reaction-based single nucleotide polymorphism (PCR-SNP) assay for competitive engraftment of mixtures of stem/progenitor cells. We used the assay to follow engraftment in immunodeficient mice of subpopulations of the stem/progenitor cells from human bone marrow referred to as either mesenchymal stem cells or marrow stromal cells (MSCs). The engraftment into adult mice without induced tissue injury was low and variable, but there was preferential engraftment of a subpopulation of rapidly self-renewing MSCs (RS-MSCs) compared with a subpopulation of slowly renewing MSCs (SR-MSCs). After intravenous infusion, there was a tendency for the cells to engraft into the hippocampal region that was previously designated a "vascular niche." Migration assays suggested that preferential engraftment of RS-MSCs was in part explained by their expression of CXCR4 and CX3R1, the receptors for SDF-1 and fractalkine.

DOI
10.1182/blood-2005-07-2701
Citation Information
R.H. Lee, S.C. Hsu, James R. Munoz, J.S. Jung, et al.. "A subset of human rapidly self-renewing marrow stromal cells preferentially engraft in mice" Blood Vol. 107 Iss. 5 (2006) p. 2153 - 2161 ISSN: 0006-4971
Available at: http://works.bepress.com/james-munoz/8/