Article
Recent advances in capillary ultrahigh pressure liquid chromatography
Journal of Chromatography A
(2017)
Abstract
In the twenty years since its initial demonstration, capillary ultrahigh pressure liquid chromatography (UHPLC) has proven to be one of most powerful separation techniques for the analysis of complex mixtures. This review focuses on the most recent advances made since 2010 towards increasing the performance of such separations. Improvements in capillary column preparation techniques that have led to columns with unprecedented performance are described. New stationary phases and phase supports that have been reported over the past decade are detailed, with a focus on their use in capillary formats. A discussion on the instrument developments that have been required to ensure that extra-column effects do not diminish the intrinsic efficiency of these columns during analysis is also included. Finally, the impact of these capillary UHPLC topics on the field of proteomics and ways in which capillary UHPLC may continue to be applied to the separation of complex samples are addressed.
Keywords
- Capillary LC,
- Ultrahigh pressure liquid chromatography,
- nanoLC,
- Stationary phases,
- LC columns,
- Proteomics
Disciplines
Publication Date
November 10, 2017
DOI
10.1016/j.chroma.2017.05.039
Citation Information
Laura E. Blue, Edward G. Franklin, Justin M. Godinho, James P. Grinias, et al.. "Recent advances in capillary ultrahigh pressure liquid chromatography" Journal of Chromatography A Vol. 1523 (2017) p. 17 - 39 ISSN: 0021-9673 Available at: http://works.bepress.com/james-grinias/8/