Article
Measurement and Modeling of Extra-Column Effects Due to Injection and Connections in Capillary Liquid Chromatography
Chromatography
(2015)
Abstract
As column volumes continue to decrease, extra-column band broadening has become an increasingly important consideration when determining column performance. Combined contributions due to the injector and connecting tubing in a capillary LC system were measured and found to be larger than expected by Taylor-Aris theory. Variance from sigma-type and tau-type broadening was isolated from eluted peaks using the Foley-Dorsey Exponentially Modified Gaussian peak fitting model and confirmed with computational fluid dynamics. It was found that the tau-type contributions were the main cause for the excessive broadening because of poorly-swept volumes at the connection between the injector and tubing. To reduce tau-type contributions (and peak tailing), a timed pinch mode could be used for analyte injection.
Keywords
- extra-column band broadening,
- injection,
- liquid chromatography,
- peak measurements
Disciplines
Publication Date
December, 2015
DOI
10.3390/chromatography2040669
Citation Information
James Grinias, Bernard Bunner, Martin Gilar and James W. Jorgenson. "Measurement and Modeling of Extra-Column Effects Due to Injection and Connections in Capillary Liquid Chromatography" Chromatography Vol. 2 Iss. 4 (2015) p. 669 - 690 ISSN: 2227-9075 Available at: http://works.bepress.com/james-grinias/3/
Creative Commons license
This work is licensed under a Creative Commons CC_BY International License.