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Article
Cells that express MyoD mRNA in the epiblast are stably committed to the skeletal muscle lineage
Journal of Cell Biology
  • Jacquelyn Gerhart, Philadelphia College of Osteopathic Medicine
  • C. Neely
  • J. Elder
  • J. Pfautz
  • J. Perlman
  • L. Narciso
  • K. Linask
  • K. Knudsen
  • Mindy George-Weinstein, Philadelphia College of Osteopathic Medicine
Document Type
Article
Publication Date
1-1-2007
Disciplines
Abstract
The epiblast of the chick embryo contains cells that express MyoD mRNA but not MyoD protein. We investigated whether MyoD-positive (MyoDpos) epiblast cells are stably committed to the skeletal muscle lineage or whether their fate can be altered in different environments. A small number of MyoD pos epiblast cells were tracked into the heart and nervous system. In these locations, they expressed MyoD mRNA and some synthesized MyoD protein. No MyoDpos epiblast cells differentiated into cardiac muscle or neurons. Similar results were obtained when MyoDpos cells were isolated from the epiblast and microinjected into the precardiac mesoderm or neural plate. In contrast, epiblast cells lacking MyoD differentiated according to their environment. These results demonstrate that the epiblast contains both multipotent cells and a subpopulation of cells that are stably committed to the skeletal muscle lineage before the onset of gastrulation. Stable programming in the epiblast may ensure that MyoDpos cells express similar signaling molecules in a variety of environments.
Comments

This article was published in Journal of Cell Biology, Volume 178, Issue 4, Pages 649-660.

The published version is available at http://dx.doi.org/10.1083/jcb.200703060.

Copyright © 2007 Rockefeller University Press.

Citation Information
Jacquelyn Gerhart, C. Neely, J. Elder, J. Pfautz, et al.. "Cells that express MyoD mRNA in the epiblast are stably committed to the skeletal muscle lineage" Journal of Cell Biology Vol. 178 Iss. 4 (2007) p. 649 - 660
Available at: http://works.bepress.com/jacquelyn-gerhart/3/