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Article
Visualizing the needle in the haystack: In situ hybridization with fluorescent dendrimers
Biological Procedures Online
  • Jacquelyn Gerhart, Philadelphia College of Osteopathic Medicine
  • M. Baytion
  • J. Perlman
  • C. Neely
  • B. Hearon
  • T. Nilsen
  • R. Getts
  • J. Kadushin
  • Mindy George-Weinstein, Philadelphia College of Osteopathic Medicine
Document Type
Article
Publication Date
1-1-2004
Disciplines
Abstract
In situ hybridization with 3DNA dendrimers is a novel tool for detecting low levels of mRNA in tissue sections and whole embryos. Fluorescently labeled dendrimers were used to identify cells that express mRNA for the skeletal muscle transcription factor MyoD in the early chick embryo. A small population of MyoD mRNA positive cells was found in the epiblast prior to the initiation of gastrulation, two days earlier than previously detected using enzymatic or radiolabeled probes for mRNA. When isolated from the epiblast and placed in culture, the MyoD mRNA positive cells were able to differentiate into skeletal muscle cells. These results demonstrate that DNA dendrimers are sensitive and precise tools for identifying low levels of mRNA in single cells and tissues.
Comments

This article was published in Biological Procedures Online, Volume 6, Issue 1, Pages 149-156.

The published version is available at http://dx.doi.org/10.1251/bpo84.

Copyright © 2004 Springer

Citation Information
Jacquelyn Gerhart, M. Baytion, J. Perlman, C. Neely, et al.. "Visualizing the needle in the haystack: In situ hybridization with fluorescent dendrimers" Biological Procedures Online Vol. 6 Iss. 1 (2004) p. 149 - 156
Available at: http://works.bepress.com/jacquelyn-gerhart/25/