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Tracking and ablating subpopulations of epiblast cells in the chick embryo
Biological Procedures Online
  • Jacquelyn Gerhart, Philadelphia College of Osteopathic Medicine
  • Christine Neely
  • J. Pfautz
  • Mindy George-Weinstein, Philadelphia College of Osteopathic Medicine
Document Type
Article
Publication Date
1-1-2008
Disciplines
Abstract
The early chick embryo contains subpopulations of cells that express lineage-specific transcription factors. We have developed protocols to examine the role of these cells during development that involve labeling them for cell tracking purposes and ablating them within the epiblast. The procedures take advantage of the fact that subpopulations of epiblast cells differentially express cell surface antigens recognized by monoclonal antibodies. Embryos are removed from the shell and incubated on the yolk with an antibody. Cells that bind the antibody are either tagged with a fluorescent secondary antibody or lysed with complement. For long-term analyses, embryos are returned to a host shell and placed in an incubator. This method of whole embryo manipulation ex-ovo and incubation in-ovo supports normal development into the fetal period.
Comments

This article was published in Biological Procedures Online, Volume 10, Issue 1, Pages 74-82.

The published version is available at http://dx.doi.org/10.1251/bpo145.

Copyright © 2008 Springer

Citation Information
Jacquelyn Gerhart, Christine Neely, J. Pfautz and Mindy George-Weinstein. "Tracking and ablating subpopulations of epiblast cells in the chick embryo" Biological Procedures Online Vol. 10 Iss. 1 (2008) p. 74 - 82
Available at: http://works.bepress.com/jacquelyn-gerhart/24/