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Article
NeuroM and MyoD are Expressed in Separate Subpopulations of Cells in the Pregastrulating Epiblast
Gene Expression Patterns
  • Robert Strony
  • Jacquelyn Gerhart, Philadelphia College of Osteopathic Medicine
  • Dolores Tornambe, Philadelphia College of Osteopathic Medicine
  • Jordanna Perlman
  • Christine Neely
  • Jeffrey Dare
  • Benjamin Stewart
  • Mindy George-Weinstein, Philadelphia College of Osteopathic Medicine
Document Type
Article
Publication Date
2-1-2005
Abstract
Epiblast cells form skeletal muscle and neurons in culture and some express mRNA for the skeletal muscle specific transcription factor MyoD in vivo. The following experiments were designed to determine whether the neurogenic transcription factor NeuroM is expressed in the epiblast and if NeuroM and MyoD are present in separate subpopulations of epiblast cells that can differentiate into neurons and muscle, respectively. In situ hybridization revealed that NeuroM was present in the anterior region of the pregastrulating epiblast. Some cells with NeuroM were proliferating and expressed two molecules present in neurogenic cells, NCAM and the Zn-12/HNK-1 carbohydrate. The G8 antibody labeled cells with MyoD but not NeuroM. When G8 positive cells were isolated by magnetic cell sorting and placed in culture, nearly all differentiated into skeletal muscle in serum free medium. A subpopulation of cells isolated with antibodies that bound to cells expressing NeuroM formed neurons when cultured in medium supplemented with sera and embryo extract. These experiments demonstrate that NeuroM and MyoD are present in separate subpopulations of cells in the pregastrulating epiblast. Epiblast cells with NeuroM are more dependent on exogenous factors to differentiate than those with MyoD.
PubMed ID
15661645
Comments

This article was published in Gene Expression Patterns, Volume 5, Issue 3, February 2005, Pages 387-95.

The published version is available at http://dx.doi.org/10.1016/j.modgep.2004.09.006

Copyright © 2005 Elsevier B.V.

Citation Information
Robert Strony, Jacquelyn Gerhart, Dolores Tornambe, Jordanna Perlman, et al.. "NeuroM and MyoD are Expressed in Separate Subpopulations of Cells in the Pregastrulating Epiblast" Gene Expression Patterns Vol. 5 Iss. 3 (2005) p. 387 - 395
Available at: http://works.bepress.com/jacquelyn-gerhart/23/