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Article
Epiblast cells that express MyoD recruit pluripotent cells to the skeletal muscle lineage
Journal of Cell Biology
  • Jacquelyn Gerhart, Philadelphia College of Osteopathic Medicine
  • C. Neely
  • B. Stewart
  • J. Perlman
  • D. Beckmann
  • M. Wallon
  • K. Knaudsen
  • Mindy George-Weinstein, Philadelphia College of Osteopathic Medicine
Document Type
Article
Publication Date
1-1-2004
Disciplines
Abstract
Embryonic stem cells are derived from the epiblast. A subpopulation of epiblast cells expresses MyoD mRNA and the G8 antigen in vivo. G8 positive (G8pos) and G8 negative (G8neg) populations were isolated by magnetic cell sorting. Nearly all G8pos cells switched from E- to N-cadherin and differentiated into skeletal muscle in culture. G8neg cells were impaired in their ability to switch cadherins and few formed skeletal muscle. Medium conditioned by G8pos cells stimulated skeletal myogenesis and N-cadherin synthesis in G8neg cultures. The effect of conditioned medium from G8pos cultures was inhibited by bone morphogenetic protein (BMP) 4. Treatment of G8neg cells with a soluble form of the BMP receptor-1A or Noggin promoted N-cadherin synthesis and skeletal myogenesis. These results demonstrate that MyoD-positive epiblast cells recruit pluripotent cells to the skeletal muscle lineage. The mechanism of recruitment involves blocking the BMP signaling pathway.
Comments

This article was published in Journal of Cell Biology, Volume 164, Issue 5, Pages 739-746.

The published version is available at http://dx.doi.org/10.1083/jcb.200309152.

Copyright © 2004 Rockefeller University Press.

Citation Information
Jacquelyn Gerhart, C. Neely, B. Stewart, J. Perlman, et al.. "Epiblast cells that express MyoD recruit pluripotent cells to the skeletal muscle lineage" Journal of Cell Biology Vol. 164 Iss. 5 (2004) p. 739 - 746
Available at: http://works.bepress.com/jacquelyn-gerhart/16/