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Article
DNA dendrimers localize MyoD mRNA in presomitic tissues of the chick embryo
Journal of Cell Biology
  • Jacquelyn Gerhart, Philadelphia College of Osteopathic Medicine
  • M. Baytion
  • S. Deluca
  • R. Getts
  • C. Lopez
  • R. Niewenhuis
  • T. Nilsen
  • S. Olex
  • H. Weintraub
  • Mindy George-Weinstein, Philadelphia College of Osteopathic Medicine
Document Type
Article
Publication Date
1-1-2000
Disciplines
Abstract
MyoD expression is thought to be induced in somites in response to factors released by surrounding tissues; however, reverse transcription-PCR and cell culture analyses indicate that myogenic cells are present in the embryo before somite formation. Fluorescently labeled DNA dendrimers were used to identify MyoD expressing cells in presomitic tissues in vivo. Subpopulations of MyoD positive cells were found in the segmental plate, epiblast, mesoderm, and hypoblast. Directly after laying, the epiblast of the two layered embryo contained ~20 MyoD positive cells. These results demonstrate that dendrimers are precise and sensitive reagents for localizing low levels of mRNA in tissue sections and whole embryos, and that cells with myogenic potential are present in the embryo before the initiation of gastrulation.
Comments

This article was published in Journal of Cell Biology, Volume 149, Issue 4, Pages 825-833.

The published version is available at http://dx.doi.org/10.1083/jcb.149.4.825.

Copyright © 2000 Elsevier.

Citation Information
Jacquelyn Gerhart, M. Baytion, S. Deluca, R. Getts, et al.. "DNA dendrimers localize MyoD mRNA in presomitic tissues of the chick embryo" Journal of Cell Biology Vol. 149 Iss. 4 (2000) p. 825 - 833
Available at: http://works.bepress.com/jacquelyn-gerhart/15/