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Addressing fluorogenic real-time qPCR inhibition using the novel custom Excel file system ‘FocusField2-6GallupqPCRSet-upTool-001’ to attain consistently high fidelity qPCR reactions
Biological Procedures Online
  • Jack M. Gallup, Iowa State University
  • Mark R. Ackermann, Iowa State University
Document Type
Article
Publication Date
12-1-2006
DOI
10.1251/bpo122
Abstract

The purpose of this manuscript is to discuss fluorogenic real-time quantitative polymerase chain reaction (qPCR) inhibition and to introduce/define a novel Microsoft Excel-based file system which provides a way to detect and avoid inhibition, and enables investigators to consistently design dynamically-sound, truly LOG-linear qPCR reactions very quickly. The qPCR problems this invention solves are universal to all qPCR reactions, and it performs all necessary qPCR set-up calculations in about 52 seconds (using a pentium 4 processor) for up to seven qPCR targets and seventytwo samples at a time - calculations that commonly take capable investigators days to finish. We have named this custom Excel-based file system “FocusField2-6GallupqPCRSet-upTool-001” (FF2-6-001 qPCR set-up tool), and are in the process of transforming it into professional qPCR set-up software to be made available in 2007. The current prototype is already fully functional.

Comments

This article is from Biological Procedures Online 8, no. 1 ( December 2006): 87–153, doi:10.1251/bpo122.

Copyright Owner
The authors
Language
en
Date Available
February 25, 2013
File Format
application/pdf
Citation Information
Jack M. Gallup and Mark R. Ackermann. "Addressing fluorogenic real-time qPCR inhibition using the novel custom Excel file system ‘FocusField2-6GallupqPCRSet-upTool-001’ to attain consistently high fidelity qPCR reactions" Biological Procedures Online Vol. 8 Iss. 1 (2006) p. 87 - 153
Available at: http://works.bepress.com/jack_gallup/8/