Greenhouse gas emissions (GHGs) from swine production systems are relatively well researched with the exception of emissions from land application of manure. GHGs inventories are needed for process-based modeling and science-based regulations. Thus, the objective of this observational study was to measure GHG fluxes from land application of swine manure on a typical corn field. Assessment of GHG emissions from deep injected land-applied swine manure, fall and reapplication in the spring, on a typical US Midwestern corn-on-corn farm was completed. Static chambers were used for flux measurement along with gas analysis on a GC-FID-ECD. Measured gas concentrations were used to estimate GHGs flux using four different models: linear regression, nonlinear regression, first order linear regression and the revised Hutchinson and Mosier (HMR) model, respectively for comparisons. Cumulative flux estimates after manure application of 5.85 × 105 g•ha–1 (1 ha = 0.01 km2) of CO2, 6.60 × 101g•ha–1 of CH4, and 3.48 × 103 g•ha–1 N2O for the fall trial and 3.11 × 106g•ha–1 of CO2, 2.95 × 103 g•ha–1 of CH4, and 1.47 × 104 g•ha–1 N2O after the spring reapplication trial were observed. The N2O net cumulative flux represents 0.595% of nitrogen applied in swine manure for the fall trial.