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Article
Efficacy of NH3 as a secondary barrier treatment for inactivation of Salmonella Typhimurium and methicillin-resistant Staphylococcus aureus in digestate of animal carcasses: Proof-of-concept
PLoS ONE
  • Jacek A. Koziel, Iowa State University
  • Timothy S. Frana, Iowa State University
  • Heekwon Ahn, Chungnam National University
  • Thomas D. Glanville, Iowa State University
  • Lam T. Nguyen, Iowa State University
  • J. (Hans) van Leeuwen, Iowa State University
Document Type
Article
Publication Version
Published Version
Publication Date
5-5-2017
DOI
10.1371/journal.pone.0176825
Abstract

Managing the disposal of infectious animal carcasses from routine and catastrophic disease outbreaks is a global concern. Recent research suggests that burial in lined and aerated trenches provides the rapid pathogen containment provided by burial, while reducing air and water pollution potential and the length of time that land is taken out of agricultural production. Survival of pathogens in the digestate remains a concern, however. A potential answer is a ‘dual’-barrier approach in which ammonia is used as a secondary barrier treatment to reduce the risk of pathogen contamination when trench liners ultimately leak. Results of this study showed that the minimum inhibitory concentration (MIC) of NH3 is 0.1 M (~1,468 NH3-N mg/L), and 0.5 M NH3 (~7,340 NH3-N mg/L) for ST4232 & MRSA43300, respectively at 24 h and pH = 9±0.1 and inactivation was increased by increasing NH3 concentration and/or treatment time. Results for digestate treated with NH3 were consistent with the MICs, and both pathogens were completely inactivated within 24 h.

Comments

This article is from PLoS ONE 12(5): e0176825. https://doi.org/10.1371/journal.pone.0176825.

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Creative Commons License
Creative Commons Attribution 4.0 International
Copyright Owner
Koziel et al.
Language
en
File Format
application/pdf
Citation Information
Jacek A. Koziel, Timothy S. Frana, Heekwon Ahn, Thomas D. Glanville, et al.. "Efficacy of NH3 as a secondary barrier treatment for inactivation of Salmonella Typhimurium and methicillin-resistant Staphylococcus aureus in digestate of animal carcasses: Proof-of-concept" PLoS ONE Vol. 12 Iss. 5 (2017) p. e0176825
Available at: http://works.bepress.com/jacek_koziel/137/