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Quantitative elucidation of a distinct spatial gradient-sensing mechanism in fibroblasts
Journal of Cell Biology (2005)
  • Ian C. Schneider, North Carolina State University at Raleigh
  • Jason M. Haugh, North Carolina State University at Raleigh
Migration of eukaryotic cells toward a chemoattractant often relies on their ability to distinguish receptor-mediated signaling at different subcellular locations, a phenomenon known as spatial sensing. A prominent example that is seen during wound healing is fibroblast migration in platelet-derived growth factor (PDGF) gradients. As in the well-characterized chemotactic cells Dictyostelium discoideum and neutrophils, signaling to the cytoskeleton via the phosphoinositide 3-kinase pathway in fibroblasts is spatially polarized by a PDGF gradient; however, the sensitivity of this process and how it is regulated are unknown. Through a quantitative analysis of mathematical models and live cell total internal reflection fluorescence microscopy experiments, we demonstrate that PDGF detection is governed by mechanisms that are fundamentally different from those in D. discoideum and neutrophils. Robust PDGF sensing requires steeper gradients and a much narrower range of absolute chemoattractant concentration, which is consistent with a simpler system lacking the feedback loops that yield signal amplification and adaptation in amoeboid cells.
  • phosphatidylinositol 3 kinase,
  • cell migration,
  • Dictyostelium discoideum,
  • feedback system,
  • fibroblast,
  • fluorescence microscopy,
  • neutrophil,
  • quantitative analysis,
  • signal transduction,
  • Platelet-Derived Growth Factor
Publication Date
December, 2005
Citation Information
Ian C. Schneider and Jason M. Haugh. "Quantitative elucidation of a distinct spatial gradient-sensing mechanism in fibroblasts" Journal of Cell Biology Vol. 171 Iss. 5 (2005)
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