Document Type
Conference Proceeding
Conference
2016 IEEE SENSORS
Publication Version
Accepted Manuscript
Link to Published Version
https://doi.org/10.1109/ICSENS.2016.7808800
Publication Date
1-1-2016
DOI
10.1109/ICSENS.2016.7808800
Conference Title
2016 IEEE SENSORS
Conference Date
Oct. 30-Nov. 3, 2016
Geolocation
(28.5383355, -81.3792365)
Abstract
This paper reports, for the first time, an optimized microfluidic droplet device for fabricating 3D microtissues and studying the cell behaviors in 3D microtissues. It has been found by properly selecting the size of the microchambers on the microfluidic device and choosing an optimal concentration of collagen (2 mg/ml) to fabricate microtissues, the behaviors of cells in the microtissues can be essentially the same as those of cells in a conventional cell culture system. The normal cell spreading and division in the microtissue have been observed, and the cell migration speed is ~14.1 μm/hr, close to that of 17.3 μm/hr in a macroscale tissue. All these experimental results suggest the microfluidic droplet device might provide a new avenue to replace other approaches to fabricate 3D microtissues and study cell behaviors.
Rights
© 2016 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works.
Citation Information
Xiangchen Che, Shenmin Gong, Long Que, Jacob Nuhn, et al.. "Studies of cell behaviors in 3D microtissues in a microfluidic device: Growth and migration" Orlando, FL
IEEE SENSORS (2016) p. 1 - 3
Available at: http://works.bepress.com/ian_schneider/31/
This is a manuscript of a proceeding published as Che, Xiangchen, Shenmin Gong, Long Que, Jacob Nuhn, and Ian Schneider. "Studies of cell behaviors in 3D microtissues in a microfluidic device: growth and migration." In 2016 IEEE SENSORS. (2016): 1-3. DOI: 10.1109/ICSENS.2016.7808800. Posted with permission.