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Article
Fermentation of Cottonseed and Other Feedstuffs in Cattle Rumen Fluid
Journal of Agricultural and Food Chemistry
  • Ian C. Schneider, Iowa State University
  • Michael L. Ames, Iowa State University
  • Mark A. Rasmussen, United States Department of Agriculture
  • Peter J. Reilly, Iowa State University
Document Type
Article
Publication Date
3-3-2002
DOI
10.1021/jf010783n
Abstract

Bovine rumen fluid was fermented anaerobically over 48 h with cottonseed, corn, alfalfa, or a mixture of these substrates in anaerobic mineral buffer. Samples taken at different incubation times were derivatized with n-butanol and subjected to gas chromatography and mass spectroscopy. No unusual fermentation end-products from the cottonseed substrate were detected. Cottonseed supported rumen fermentation at levels comparable to those of the other substrates. Major components were usually found in the decreasing order of acetate, propionate, butyrate, and valerate, although acetate and propionate concentrations decreased late in the alfalfa and mixed-feed fermentations, eventually allowing butyrate concentrations to exceed those of propionate. As expected, lactate was produced in high concentrations when corn was fermented. The minor components 2-methylpropionate, 2- and 3-methylbutyrate, phenylacetate, phenylpropionate, and caproate also accumulated, with their relative concentrations varying with the substrate. Succinate was produced in substantial amounts only when corn and alfalfa were fermented; it did not accumulate when cottonseed was the substrate. Samples containing cottonseed were derivatized and subjected to reversed-phase high-performance liquid chromatography, revealing that gossypol concentrations did not change during fermentation.

Comments

Posted with permission from Journal of Agricultural and Food Chemistry, 50, no. 8 (2002): 2267–2273, doi:10.1021/jf010783n. Copyright 2002 American Chemical Society.

Copyright Owner
American Chemical Society
Language
en
Date Available
November 10, 2012
File Format
application/pdf
Citation Information
Ian C. Schneider, Michael L. Ames, Mark A. Rasmussen and Peter J. Reilly. "Fermentation of Cottonseed and Other Feedstuffs in Cattle Rumen Fluid" Journal of Agricultural and Food Chemistry Vol. 50 Iss. 8 (2002) p. 2267 - 2273
Available at: http://works.bepress.com/ian_schneider/1/