In recent years, researchers pay attention to simple and affordable paper based biosensor as the cost of biosensors and lack of equipment are obstacles for early detection of diseases. The paper based biosensor has advantages to become a point-of-care diagnostic platform especially in developing countries as it requires minimal equipment or trained individuals to perform the test. The biosensor can be prepared in a paper via inexpensive wax printing and run the sample fluid without a pump. The detection can be colorimetric so that the pattern can be identified by naked eyes or with the help of smart phone camera and app system. We are taking a first step into development of paper based biosensor exploring different fluid channel patterns and detection scheme. The model detection system is developed for prostate cancer antigen (PSA) that is an early biomarker for prostate cancer and is present in blood serum at an elevated level of 100 ng/ml for onset of the cancer. Antibodies are attached to 40 nm gold nanoparticle to deliver a clear red-purple detection mark when an elevated level of PSA is present in the sample.
Available at: http://works.bepress.com/hyun_kwon/15/