Skip to main content
Article
Transformation of non-cancerous human breast epithelial cell line MCF10A by the tobacco-specific carcinogen NNK
Breast Cancer Research and Treatment (2003)
  • J Mei
  • H Hu
  • Michael McEntee, University of Tennessee, Knoxville
  • Howard Plummer, 3rd, University of Tennessee, Knoxville
  • P Song
  • Hwa-Chain Robert Wang, University of Tennessee, Knoxville
Abstract
Repeated treatments of non-cancerous human breast epithelial cells MCF10A with a low dose of the tobacco-specific carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) induced the development of cancerous cells. NNK-transformed MCF10A cells acquired cancerous properties including anchorage-independent cell growth and increased cell motility. Cellular transformation of MCF10A cells was accompanied by a loss of responsiveness to 17beta-estradiol and decreased rate of cell proliferation. NNK-transformed MCF10A cells were also tumorigenic in immunodifficient mice. Studies of changes in the regulation of intracellular signaling pathways revealed that the upstream Erk pathway was down-regulated in the NNK-transformed cells. Our data provide the first evidence suggesting that the tobacco carcinogen NNK is competent to induce malignant transformation of non-cancerous human breast epithelial cells. Our findings suggest that the tobacco carcinogen NNK may contribute to early events in human breast carcinogenesis.
Publication Date
2003
Citation Information
J Mei, H Hu, Michael McEntee, Howard Plummer, et al.. "Transformation of non-cancerous human breast epithelial cell line MCF10A by the tobacco-specific carcinogen NNK" Breast Cancer Research and Treatment Vol. 79 Iss. 1 (2003)
Available at: http://works.bepress.com/hwa-chain_wang/16/