Article
Functional characterization of bursicon receptor and genome-wide analysis for identification of genes affected by bursicon receptor RNAi
Developmental Biology
(2010)
Abstract
Bursicon is an insect neuropeptide hormone that is secreted from the central nervous system into the hemolymph and initiates cuticle tanning. The receptor for bursicon is encoded by the rickets (rk) gene and belongs to the G protein-coupled receptor (GPCR) superfamily. The bursicon and its receptor regulate cuticle tanning as well as wing expansion after adult eclosion. However, the molecular action of bursicon signaling remains unclear. We utilized RNA interference (RNAi) and microarray to study the function of the bursicon receptor (Tcrk) in the model insect, Tribolium castaneum. The data included here showed that in addition to cuticle tanning and wing expansion reported previously, Tcrk is also required for development and expansion of integumentary structures and adult eclosion. Using custom microarrays, we identified 24 genes that are differentially expressed between Tcrk RNAi and control insects. Knockdown in the expression of one of these genes, TC004091, resulted in the arrest of adult eclosion. Identification of genes that are involved in bursicon receptor mediated biological processes will provide tools for future studies on mechanisms of bursicon action.
Keywords
- Tanning,
- wing expansion,
- ecdysis,
- RNAi,
- microarray,
- Tribolium castaneum
Disciplines
Publication Date
August, 2010
DOI
10.1016/j.ydbio.2010.05.003
Publisher Statement
NOTICE: this is the author’s version of a work that was accepted for publication in Developmental Biology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Developmental Biology, VOL 344, ISSUE 1, 2010, DOI: 10.1016/j.ydbio.2010.05.003
Citation Information
Hua Bai and Subba R. Palli. "Functional characterization of bursicon receptor and genome-wide analysis for identification of genes affected by bursicon receptor RNAi" Developmental Biology Vol. 344 Iss. 1 (2010) p. 248 - 258 Available at: http://works.bepress.com/hua-bai/14/
Creative Commons license
This work is licensed under a Creative Commons CC_BY-NC-ND International License.