An effective and efficient e-Learning platform should hide systems' complexity and provide an easy and flexible interaction operation. Poor interface design of e-Learning platform is a main factor impacting on students' interactive with system and learning outcomes. The purpose of this study is to evaluate a non-degree e-Learning platform with Performance Analysis and User perception methods to investigate the system's efficiency, effectiveness and satisfaction. In this study, efficiency and effectiveness are evaluated by Time on Task and Task Completion score. Satisfaction is evaluated by Questionnaire and participants' feedback. Based on these data, this article analyzed the main interface design problem that frustrate learners and distract them from learning task, and given some advice for interface design in e-Learning platform.