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SNP-based high density genetic map and mapping of btwd1 dwarfing gene in barley
Scientific Reports
  • Xifeng Ren, Huazhong Agricultural University
  • Jibin Wang, Huazhong Agricultural University
  • Lipan Liu, Huazhong Agricultural University
  • Genlou Sun, Saint Mary’s University
  • Chengdao Li, Department of Agriculture & Food/Agricultural Research Western Australia
  • Hong Luo, Clemson University
  • Dongfa Sun, Huazhong Agricultural University
Document Type
Article
Publication Date
8-1-2016
Publisher
Nature Publishing Group
Disciplines
Abstract

A high-density linkage map is a valuable tool for functional genomics and breeding. A newly developed sequence-based marker technology, restriction site associated DNA (RAD) sequencing, has been proven to be powerful for the rapid discovery and genotyping of genome-wide single nucleotide polymorphism (SNP) markers and for the high-density genetic map construction. The objective of this research was to construct a high-density genetic map of barley using RAD sequencing. 1894 high-quality SNP markers were developed and mapped onto all seven chromosomes together with 68 SSR markers. These 1962 markers constituted a total genetic length of 1375.8 cM and an average of 0.7 cM between adjacent loci. The number of markers within each linkage group ranged from 209 to 396. The new recessive dwarfing gene btwd1 in Huaai 11 was mapped onto the high density linkage maps. The result showed that the btwd1 is positioned between SNP marks 7HL_6335336 and 7_249275418 with a genetic distance of 0.9 cM and 0.7 cM on chromosome 7H, respectively. The SNP-based high-density genetic map developed and the dwarfing gene btwd1 mapped in this study provide critical information for position cloning of the btwd1gene and molecular breeding of barley.

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The published version can be found here: http://www.nature.com/articles/srep31741

DOI: 10.1111/pbi.12638

Citation Information
Ren, X. et al. SNP-based high density genetic map and mapping of btwd1 dwarfing gene in barley. Sci. Rep. 6, 31741; doi: 10.1038/srep31741 (2016).