Antigen presenting properties of a myeloid dendritic-like cell in murine spleenPL o S One
Date of this Version9-21-2016
Document TypeJournal Article
Grant NumberNational Health and Medical Research Council of Australia to H.O (Project grant # 585443)
Distribution LicenseCreative Commons Attribution 4.0
AbstractThis paper distinguishes a rare subset of myeloid dendritic-like cells found in mouse spleen from conventional (c) dendritic cells (DC) in terms of phenotype, function and gene expression. These cells are tentatively named “L-DC” since they resemble dendritic-like cells produced in longterm cultures of spleen. L-DC can be distinguished on the basis of their unique phenotype as CD11bhiCD11cloMHCII-CD43+Ly6C-Ly6G-Siglec-F- cells. They demonstrate similar ability as cDC to uptake and retain complex antigens like mannan via mannose receptors, but much lower ability to endocytose and retain soluble antigen. While L-DC differ from cDC by their inability to activate CD4+ T cells, they are capable of antigen cross-presentation for activation of CD8+ T cells, although less effectively so than the cDC subsets. In terms of gene expression, CD8- cDC and CD8+ cDC are quite distinct from L-DC. CD8+ cDC are distinguishable from the other two subsets by expression of CD24a, Clec9a, Xcr1 and Tlr11, while CD8- cDC are distinguished by expression of Ccnd1 and H-2Eb2. L-DC are distinct from the two cDC subsets through upregulated expression of Clec4a3, Emr4, Itgam, Csf1r and CD300ld. The L-DC gene profile is quite distinct from that of cDC, confirming a myeloid cell type with distinct antigen presenting properties.
Citation InformationYing-ying Hey and Helen C O'Neill. "Antigen presenting properties of a myeloid dendritic-like cell in murine spleen" PL o S One Vol. 11 Iss. 9 (2016) p. 1 - 23 ISSN: 1932-6203
Available at: http://works.bepress.com/helen_oneill/47/