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Article
Antigen presenting properties of a myeloid dendritic-like cell in murine spleen
PL o S One
  • Ying-ying Hey, Bond University, Queensland, Australia
  • Helen C O'Neill, Bond University
Date of this Version
9-21-2016
Document Type
Journal Article
Grant Number
National Health and Medical Research Council of Australia to H.O (Project grant # 585443)
Publication Details

Published version

Hey, Y-y., O’Neill, H.C. (2016). Antigen presenting properties of a myeloid dendritic-like cell in murine spleen. PLoS ONE, 11(9), e0162358

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Copyright © 2016 Hey, O’Neill

Distribution License
Creative Commons Attribution 4.0
Abstract

This paper distinguishes a rare subset of myeloid dendritic-like cells found in mouse spleen from conventional (c) dendritic cells (DC) in terms of phenotype, function and gene expression. These cells are tentatively named “L-DC” since they resemble dendritic-like cells produced in longterm cultures of spleen. L-DC can be distinguished on the basis of their unique phenotype as CD11bhiCD11cloMHCII-CD43+Ly6C-Ly6G-Siglec-F- cells. They demonstrate similar ability as cDC to uptake and retain complex antigens like mannan via mannose receptors, but much lower ability to endocytose and retain soluble antigen. While L-DC differ from cDC by their inability to activate CD4+ T cells, they are capable of antigen cross-presentation for activation of CD8+ T cells, although less effectively so than the cDC subsets. In terms of gene expression, CD8- cDC and CD8+ cDC are quite distinct from L-DC. CD8+ cDC are distinguishable from the other two subsets by expression of CD24a, Clec9a, Xcr1 and Tlr11, while CD8- cDC are distinguished by expression of Ccnd1 and H-2Eb2. L-DC are distinct from the two cDC subsets through upregulated expression of Clec4a3, Emr4, Itgam, Csf1r and CD300ld. The L-DC gene profile is quite distinct from that of cDC, confirming a myeloid cell type with distinct antigen presenting properties.

Citation Information
Ying-ying Hey and Helen C O'Neill. "Antigen presenting properties of a myeloid dendritic-like cell in murine spleen" PL o S One Vol. 11 Iss. 9 (2016) p. 1 - 23 ISSN: 1932-6203
Available at: http://works.bepress.com/helen_oneill/47/