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Article
Identification of a novel antigen cross-presenting cell in spleen: a counterpart to cells produced in long-term culture
Journal of Cellular and Molecular Medicine (2011)
  • Jonathan Tan, Australian National University
  • Ben Quah, Australian National University
  • Kristin Griffiths, Australian National University
  • Pravin Periasamy, Australian National University
  • Yingying Hey, Australian National University
  • Helen O'Neill, Australian National University
Abstract
Antigen-presenting cells (APC), like dendritic cells (DC), are essential for T-cell activation, leading to immunity or tolerance. Multiple DC subsets each play a unique role in the immune response. Here, a novel splenic dendritic-like APC has been characterized in mice that has immune function and cell surface phenotype distinct from other, described DC subsets. These were identified as a cell type continuously produced in spleen long-term cultures (LTC) and have anin vivoequivalent cell type in mice, namely 'L-DC'. This study characterizes LTC-DC in terms of marker phenotype and function, and compares them with L-DC and other known splenic DC and myeloid subsets. L-DC display a myeloid dendritic-like phenotype equivalent to LTC-DC as CD11c loCD11b hiMHC-II -CD8α - cells, distinct by high accessibility and endocytic capacity for blood-borne antigen. Both LTC-DC and L-DC have strong antigen cross-presentation ability leading to strong activation of CD8 + T cells, particularly after exposure to lipopolysaccharide. However, they have weak ability to stimulate CD4 + T cells in antigen-specific responses. Evidence is presented here for a novel DC type produced byin vitrohaematopoiesis which has distinct antigen-presenting potential and reflects a DC subset present alsoin vivoin spleen.
Publication Date
2011
Publisher Statement
© Copyright, The Authors & Journal of Cellular and Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd, 2011
Citation Information
Jonathan Tan, Ben Quah, Kristin Griffiths, Pravin Periasamy, et al.. "Identification of a novel antigen cross-presenting cell in spleen: a counterpart to cells produced in long-term culture" Journal of Cellular and Molecular Medicine Vol. 15 Iss. 5 (2011)
Available at: http://works.bepress.com/helen_oneill/3/