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Article
Characterisation of dendritic cells arising from progenitors endogenous to murine spleen
PLoS ONE (2014)
  • Sawang Petvises
  • Helen O'Neill, Bond University
Abstract
Heterogeneity amongst dendritic cell (DC) subsets leads to a spectrum of immune response capacity against pathogens. Several DC subsets in spleen have been described which differ in terms of phenotype and function. We have previously reported a distinct population of CD11cloCD11b hiMHC-II-CD8- dendritic-like "L-DC" in murine spleen, which can also be generated in splenic stromal longterm cultures. Here, the ontogeny of L-DC development in perinatal mice has been compared with other known splenic DC subsets. Flow cytometric analysis has revealed the presence of L-DC at embryonic age (E)18.5 spleen, while plasmacytoid (p)DC and conventional (c)DC appear at 2 and 4 days following birth. Co-cultures of E18.5 spleen above splenic stroma also showed production of only L-DC, while spleen cells from D0 through D5 neonates showed production of both L-DC and cDC-like cells. Addition of an M-CSFR inhibitor to co-cultures revealed that while the development of cDC-like cells depended on M-CSF, many L-DC developed independently of M-CSF. Furthermore, purified hematopoietic stem cells (HSC) and multipotential progenitors (MPP) isolated from neonatal D1 spleen are capable of developing into L-DC in co-cultures. These studies reveal a lineage of dendritic-like cells developing in the spleen microenvironment, and which appear to arise from endogenous progenitors laid down in spleen during embryogenesis.
Keywords
  • animal,
  • cell,
  • maturation,
  • embryo
Publication Date
February 14, 2014
Citation Information
Sawang Petvises and Helen O'Neill. "Characterisation of dendritic cells arising from progenitors endogenous to murine spleen" PLoS ONE Vol. 9 Iss. 2 (2014)
Available at: http://works.bepress.com/helen_oneill/15/