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Development of two distinct dendritic-like APCs in the context of splenic stroma
Frontiers in Immunology (2013)
  • Pravin Periasamy, Australian National University
  • Sawang Petvises, Australian National University
  • Helen O'Neill, Australian National University
Abstract
Murine splenic stroma has been found to provide an in vitro niche for hematopoiesis of dendritic-like APC. Two distinct cell types have been characterized. The novel "L-DC" subset has cross-presenting capacity, leading to activation of CD8+ T cells, but not activating CD4+ T cells, which is consistent with their CD11cloCD11bhiMHC-II- phenotype. For L-DC, an equivalent tissue-specific APC has been found only in spleen. A second population of CD11chiCD11bloMHC-II+ cells resembling conventional dendritic cells (cDC) can activate both CD4 and CD8 T cells. Production of L-DC but not cDC-like cells is now shown to be dependent on contact between the L-DC progenitor and stroma such that the presence of a Transwell membrane can prevent L-DC development. Since L-DC can be produced continuously in vitro in stromal co-cultures overlaid with bone marrow (BM) progenitors, it was hypothesized that L-DC progenitors are self-renewing. The L-DC progenitor is shown here to be defined by the Flt3-c-kit+Lin-Sca-1+ (F-KLS) subset of adult BM which contains primitive HSC. Since the less primitive F+KLS HSC subset also contains L-DC progenitors, Flt3 does not appear to be a defining marker for this progenitor. Precursors of the cDC-like subset are found only within the F+KLS subset and seed production of a transient population of APC. All data identify differentiation of L-DC from HSC, and of cDC-like cells from DC precursors, which occurs independently of inflammatory signals and is dependent on a splenic stromal microenvironment.
Keywords
  • dendritic,
  • Hematopoiesis,
  • Hematopoietic,
  • stem,
  • cells,
  • stroma
Publication Date
March, 2013
Publisher Statement
© Copyright, The Authors , 2013 This work is licensed under a Creative Commons 3.0 License
Citation Information
Pravin Periasamy, Sawang Petvises and Helen O'Neill. "Development of two distinct dendritic-like APCs in the context of splenic stroma" Frontiers in Immunology Vol. 4 Iss. 73 (2013)
Available at: http://works.bepress.com/helen_oneill/12/