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Isolation, Characterization, and Chromosomal Mapping of the Human Nkx6.1 Gene (NKX6A), a New Pancreatic Islet Homeobox Gene
1990-1999
  • Helen Donis-Keller, Franklin W. Olin College of Engineering
  • Hiroshi Inoue
  • Abraham Rudnick
  • Michael S German
  • Rosalie A. Veile
  • M. Alan Permutt
Document Type
Article
Publication Date
3-1-1997
Disciplines
Abstract
Nkx6.1 (gene symbol NKX6A), a new member of the NK homeobox gene family, was recently identified in rodent pancreatic islet β-cell lines. The pattern of expression suggested that this gene product might be important for control of islet development and/or regulation of insulin biosynthesis. We now report cloning of human NKX6A, characterization of its genomic structure, and its chromosomal localization. The predicted protein of human NKX6A contained 367 amino acids and had 97% identity to the hamster protein. The highly conserved NK decapeptide and homeodomain regions were identical between human and hamster, suggesting functional importance of these domains. The coding region spanned approximately 4.8 kb and was composed of three exons. The gene was localized to four CEPH “B” yeast artificial chromosome clones (914B4, 951G9, 981D6, and 847B3), and a nearby polymorphic marker (D4S1538) on chromosome 4 was identified fluorescencein situhybridization, we also determined that NKX6A maps to 4q21.2–q22.
Comments

© 1997 Elsevier. This article was published in Genomics, vol. 40, iss. 2, p. 367-370 and may be found here.

Citation Information
Helen Donis-Keller, Hiroshi Inoue, Abraham Rudnick, Michael S German, et al.. "Isolation, Characterization, and Chromosomal Mapping of the Human Nkx6.1 Gene (NKX6A), a New Pancreatic Islet Homeobox Gene" (1997)
Available at: http://works.bepress.com/helen_donis-keller/21/