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Article
Effects of Time, Temperature, and Storage Container on the Growth of Fusarium Species: Implications for the Worldwide Fusarium Keratitis Epidemic of 2004-2006
Archives of Ophthalmology
  • John D. Bullock, Wright State University - Main Campus
  • B. Laurel Elder, Wright State University - Main Campus
  • Harry J. Khamis, Wright State University - Main Campus
  • Ronald E. Warwar, Wright State University - Main Campus
Document Type
Article
Publication Date
2-4-2011
Abstract
Objective: To demonstrate the effects of time, temperature, and container properties on the ability of ReNu with MoistureLoc (ReNuML; contains the antimicrobial agent alexidine) to inhibit growth of Fusarium species. Methods: ReNu with MoistureLoc was stored in its Bausch & Lomb (Rochester, New York) plastic or similarly sized glass containers for 1 and 4 weeks at room temperature, 42°C, and 56°C, and then tested for its ability to inhibit growth of 7 Fusarium isolates. Results: ReNu with MoistureLoc stored in glass containers for 1 or 4 weeks at all 3 temperatures demonstrated no significant fungistatic deterioration. However, ReNuML stored at 56°C in its Bausch & Lomb plastic container demonstrated a statistically significant fungistatic deterioration compared with room temperature storage in its original plastic container or with glass container storage at any temperature. Conclusion: When exposed to elevated storage temperature, it appears that an interaction between ReNuML and its Bausch & Lomb plastic container adversely affects the fungistatic properties of ReNuML, which could have contributed to the Fusarium keratitis epidemic of 2004 through 2006.
DOI
10.1001/archophthalmol.2010.338
Citation Information
John D. Bullock, B. Laurel Elder, Harry J. Khamis and Ronald E. Warwar. "Effects of Time, Temperature, and Storage Container on the Growth of Fusarium Species: Implications for the Worldwide Fusarium Keratitis Epidemic of 2004-2006" Archives of Ophthalmology Vol. 129 Iss. 2 (2011) p. 133 - 136 ISSN: 0003-9950
Available at: http://works.bepress.com/harry_khamis/144/