Markers linked to male-sterile, female-fertile loci on the soybean [Glycine max (L.) Merr.] molecular map would facilitate early identification of male-sterile plants. The objectives were to verify the chromosome location of the ms2 (A00–63) mutation, to determine the allelism and the chromosome location of the suspected ms2 [ms? (A00–39)] mutation, and to determine the chromosome location of the ms9 (T359) mutation. Simple sequence repeat (SSR) markers were used to molecularly map the male-sterile, female-fertile loci reported in this study. Segregating F2 populations were developed from crosses of ms?ms? (A00–39) × ‘Minsoy’ (PI 278901), ms2ms2 (A00–63) × Minsoy, and Minsoy × Ms9ms9 (T359H). The ms?(A00–39) locus was positioned on MLG O at 9.9 centiMorgans (cM) distance from the marker Sat_190. The ms2 (A00–63) locus was positioned on molecular linkage group (MLG) O between markers Sat_190 and Scaa001, with a distance of 6.9 and 9.0 cM, respectively. Thems9 locus was located on MLG N between markers Satt521 and Satt237, with a distance of 8.5 and 16.2 cM, respectively. Classical allelism tests confirmed that mutant ms? (A00–39) was allelic to ms2 (A00–63). The A00–39 mutant line was assigned Genetic Type Collection number T375H and gene symbol Ms2ms2 (Ames 2). Thus Genetic Type T360H, previously identified at Ames, Iowa, becomes Ms2ms2 (Ames 1).