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Article
Determination of the Gelation Mechanism of Freeze–Thawed Hen Egg Yolk
Journal of Agricultural and Food Chemistry
Document Type
Article
Disciplines
Publication Version
Published Version
Publication Date
11-2-2015
DOI
10.1021/acs.jafc.5b04109
Abstract
A study of yolks stored up to 168 d at −20 °C was conducted to determine the gelation behavior and mechanism of freeze–thawed yolk. Methods used were rheology, native and sodium dodecyl sulfate polyacrylamide gel electrophoresis (native- and SDS-PAGE), differential scanning calorimetry (DSC), transmission electron microscopy (TEM), particle size analysis, and proton nuclear magnetic resonance (1H NMR) spectroscopy for matrix mobility. Results indicate that both constituents of plasma and granules contributed to gelation of yolk under freezing. PAGE analyses suggest that granular proteins participated in aggregation during freeze–thaw. Increasing gel strength and particle size and decreasing water and lipid–water mobility indicate that lipoproteins or apolipoproteins aggregated. At storage times ≥84 d, increased protein and lipid mobility, the detection of smaller particles, and secondarily increased gel strength suggest the liberation of protein or lipoprotein components from previously formed aggregates and further aggregation of these constituents. Disruption of the gelled yolk matrix observed with TEM supported that ice crystal formation was required for gelation to occur. A two-stage dynamic gelation model is thus proposed.
Copyright Owner
American Chemical Society
Copyright Date
2015
Language
en
File Format
application/pdf
Citation Information
Carmen Au, Nuria C. Acevedo, Harry T. Horner and Tong Wang. "Determination of the Gelation Mechanism of Freeze–Thawed Hen Egg Yolk" Journal of Agricultural and Food Chemistry Vol. 63 Iss. 46 (2015) p. 10170 - 10180 Available at: http://works.bepress.com/harry-horner/2/
Reprinted (adapted) with permission from Journal of Agricultural and Food Chemistry, 63(46); 10170-10180. Doi: 10.1021/acs.jafc.5b04109. Copyright 2015 American Chemical Society.