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Article
Antioxidant and antibacterial activities of flavonoids and curcuminoids from Zingiber spectabile Griff
Food Control (2013)
  • Halijah Ibrahim, University of Malaya
  • Sulaiman S. F.
  • Ooi K. L.
  • Sivasothy Y.
Abstract
Monoclonal anti-tubulin antibodies were used to label microtubules in the connecting cilia and outer segments of retinal photoreceptors isolated from Rana pipiens. In paraformaldehyde-fixed rods from frogs maintained on diurnal light cycles, the anti-tubulin labeling of ciliary microtubules (mean length = 27 micron) typically extends to slightly over half the length of the outer segments (mean length = 46 micron). Rod outer segments from frogs kept in constant darkness for 3-4 weeks are longer (mean length = 53 micron) than rod outer segments from frogs maintained in cyclic lighting. However, the distribution of fractional lengths of anti-tubulin labeling of ciliary microtubules is the same for both lighting regimens. Incubating retinas in 1.0 mM colchicine prior to outer-segment fixation has no effect on the length of immunolabeling of ciliary microtubules, suggesting that post-mortem elongation artifacts are not significant. Incubating retinas in 10 microM taxol prior to fixation significantly increases the length of stained ciliary microtubules, suggesting that taxol either promotes post-mortem assembly of microtubules, or that taxol reduces post-mortem disassembly. The mean position of the end of anti-tubulin-labeled ciliary microtubules does not correspond to the position of disk shedding for any of the experimental conditions employed.
Keywords
  • Alkaloids/pharmacology,
  • Animals,
  • Cilia/ultrastructure,
  • Darkness,
  • Microtubules/drug effects/*ultrastructure,
  • Paclitaxel,
  • Photoreceptor Cells/*ultrastructure,
  • Rana pipiens,
  • Rod Cell Outer Segment/*ultrastructure,
  • Tubulin/immunology
Publication Date
2013
Citation Information
Halijah Ibrahim, Sulaiman S. F., Ooi K. L. and Sivasothy Y.. "Antioxidant and antibacterial activities of flavonoids and curcuminoids from Zingiber spectabile Griff" Food Control Vol. 30 Iss. 2 (2013)
Available at: http://works.bepress.com/halijah_ibrahim/10/