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Simple one-medium formulation regeneration of fingerroot Boesenbergia rotunda (L.) Mansf. Kulturpfl. via somatic embryogenesis
In Vitro Cellular & Developmental Biology-Plant (2005)
  • Halijah Ibrahim, University of Malaya
  • Pippen R.
  • Yusof R.
  • Tan S. K.
  • Rahman N.
  • Khalid N.
Abstract
Most published protocols necessitate different media formulations for multistep somatic embryogenesis. This study aims to establish a simple but effective formulation for the regeneration of plantlets of the pharmaceutically active Boesenbergia rotunda (L.) Mansf. Kulturpfl, formerly Boesenbergia/Kaempferia pandurata (Schult), to ensure a superior and consistent supply of materials for commercialization purposes. In this study, a single-medium formulation of Murashige and Skoog (MS) supplemented with 13.54 mu M 2,4-dichlorophenoxyacetic acid (2,4-D) was found to be the only medium out of eight formulations to promote the complete somatic embryogenesis process for the culture of B. rotunda (L.). Callus cultures were initiated from a total of 280 explants of rhizome meristem. The percentage of cultures forming embryogenic callus was 23.3 +/- 4.3% on this MS medium augmented by 13.54 mu M 2,4-D. The best plantlet regeneration rate was attained from the first subcultured callus with a mean of 6.6 +/- 0.1 plantlets per 1 cm diameter aggregate of callus. Somatic embryogenesis characteristic of monocots was evident from histological studies. The regenerated plantlets have been successfully established in soil.
Keywords
  • auxins,
  • micropropagation,
  • ginger,
  • zingiberaceae,
  • plantlet regeneration,
  • kaempferia-pandurata,
  • ginger,
  • identification,
  • constituents,
  • flavonoids,
  • cultures,
  • callus,
  • cells
Publication Date
2005
Citation Information
Halijah Ibrahim, Pippen R., Yusof R., Tan S. K., et al.. "Simple one-medium formulation regeneration of fingerroot Boesenbergia rotunda (L.) Mansf. Kulturpfl. via somatic embryogenesis" In Vitro Cellular & Developmental Biology-Plant Vol. 41 Iss. 6 (2005)
Available at: http://works.bepress.com/halijah_ibrahim/1/