Bifunctional oxidative and acidic catalysts were prepared by dispersion of zirconium sulfate at various loading (2–20 wt%) on the titanium silicalite (TS-1) using wet impregnation method. The solids were calcined at 500oC before characterizing the acidity by infrared spectroscopy technique using pyridine as a probe base molecule. Results showed that both Lewis and Bronsted acidities present in the sample. However the Bronsted acid sites are only present in samples with high zirconia sulfate loading, i.e. 15 and 20 wt%, which equal to 1.76 and 2.28 Zr4+/nm2 TS-1, respectively. The formation of Bronsted acid sites is due to the presence of disulfate species on the surface of TS-1, in which based on XRD data, sample contains double layer of zirconium.