SelectedWorks - Gunes Uzer

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Selected Works of Gunes Uzer

Assistant Professor, Department of Mechanical & Biomedical Engineering

Dr. Gunes Uzer joined the Department of Mechanical & Biomedical Engineering at Boise State in 2016 and is currently an Assistant Professor. Previously, he worked as a research associate in the Department of Medicine at the University of North Carolina – Chapel Hill. Dr. Uzer obtained his Ph.D. in Biomedical Engineering in 2013, and his M.S. in Mechanical Engineering in 2008 both from Stony Brook University in New York. His work focuses on defining the mechanical control of stem cell structure, function, and fate. He was recently awarded a National Space Biomedical Research Institute Fellowship to investigate the effects of microgravity on cell nucleoskeleton and received funding from The Scientific and Technological Research Council of Turkey to study stem cell aging.

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About Gunes Uzer

Dr. Gunes Uzer joined the Department of Mechanical & Biomedical Engineering at Boise State in 2016 and is currently an Assistant Professor. Previously, he worked as a research associate in the Department of Medicine at the University of North Carolina – Chapel Hill. Dr. Uzer obtained his Ph.D. in Biomedical Engineering in 2013, and his M.S. in Mechanical Engineering in 2008 both from Stony Brook University in New York. His work focuses on defining the mechanical control of stem cell structure, function, and fate. He was recently awarded a National Space Biomedical Research Institute Fellowship to investigate the effects of microgravity on cell nucleoskeleton and received funding from The Scientific and Technological Research Council of Turkey to study stem cell aging.

Articles (19)

Data-Driven and Cell-Specific Determination of Nuclei-Associated Actin Structure (Nina Nikitina, Nurbanu Bursa, Matthew Goelzer, et al.) Small Structures (2024)

Quantitative volumetric assessment of filamentous actin (F-actin) fibers remains challenging due to their interconnected nature, leading researchers to utilize threshold-based or qualitative measurement methods with poor reproducibility. Herein, a novel machine learning-based methodology is introduced ...

Architectural Control of Mesenchymal Stem Cell Phenotype Through Nuclear Actin (Janet Rubin, Andre J. Van Wijnen and Gunes Uzer) Nucleus (2022)

There is growing appreciation that architectural components of the nucleus regulate gene accessibility by altering chromatin organization. While nuclear membrane connector proteins link the mechanosensitive actin cytoskeleton to the nucleoskeleton, actin’s contribution to the inner ...

Atomic Force Microscopy Cantilever-Based Nanoindentation: Mechanical Property Measurements at the Nanoscale in Air and Fluid (Ashton E. Enrriques, Sean Howard, Raju Timsina, et al.) Journal of Visualized Experiments (2022)

An atomic force microscope (AFM) fundamentally measures the interaction between a nanoscale AFM probe tip and the sample surface. If the force applied by the probe tip and its contact area with the sample can ...

Nuclear Envelope Mechanobiology: Linking the Nuclear Structure and Function (Matthew Goelzer, Julianna Goelzer, Matthew L. Ferguson, et al.) Nucleus (2021)

The nucleus, central to cellular activity, relies on both direct mechanical input as well as its molecular transducers to sense external stimuli and respond by regulating intra-nuclear chromatin organization that determines cell function and fate. ...

At the Nuclear Envelope of Bone Mechanobiology (Scott Birks and Gunes Uzer) Bones (2021)

The nuclear envelope and nucleoskeleton are emerging as signaling centers that regulate how physical information from the extracellular matrix is biochemically transduced into the nucleus, affecting chromatin and controlling cell function. Bone is a mechanically ...

Lamin A/C is Dispensable to Mechanical Repression of Adipogenesis (Matthew Goelzer and Gunes Uzer) International Journal of Molecular Sciences (2021)

Mesenchymal stem cells (MSCs) maintain the musculoskeletal system by differentiating into multiple lineages, including osteoblasts and adipocytes. Mechanical signals, including strain and low-intensity vibration (LIV), are important regulators of MSC differentiation via control exerted through ...

Low-Intensity Vibration Restores Nuclear YAP Levels and Acute YAP Nuclear Shuttling in Mesenchymal Stem Cells Subjected to Simulated Microgravity (Matthew Thompson, Kali Woods, Joshua Newberg, et al.) NPJ Microgravity (2020)

Reducing the musculoskeletal deterioration that astronauts experience in microgravity requires countermeasures that can improve the effectiveness of otherwise rigorous and time-expensive exercise regimens in space. The ability of low-intensity vibrations (LIV) to activate force-responsive signaling ...

Modeling Stem Cell Nucleus Mechanics Using Confocal Microscopy (Zeke Kennedy, Joshua Newberg, Matthew Goelzer, et al.) Biomechanics and Modeling in Mechanobiology (2020)

Nuclear mechanics is emerging as a key component of stem cell function and differentiation. While changes in nuclear structure can be visually imaged with confocal microscopy, mechanical characterization of the nucleus and its sub-cellular components ...

Isolated Nuclei Stiffen in Response to Low Intensity Vibration (Joshua Newberg, Jesse Schimpf, Kali Woods, et al.) Journal of Biomechanics (2020)

The nucleus, central to all cellular activity, relies on both direct mechanical input and its molecular transducers to sense and respond to external stimuli. While it has been shown that isolated nuclei can adapt to ...

Low Intensity Vibrations Augment Mesenchymal Stem Cell Proliferation and Differentiation Capacity During in vitro Expansion (Guniz Bas, Stacie Loisate, Stephanie F. Hudon, et al.) Scientific Reports (2020)

A primary component of exercise, mechanical signals, when applied in the form of low intensity vibration (LIV), increases mesenchymal stem cell (MSC) osteogenesis and proliferation. While it is generally accepted that exercise effectively combats the ...

Center of Biomedical Research Excellence in Matrix Biology: Building Research Infrastructure, Supporting Young Researchers, and Fostering Collaboration (Julia Thom Oxford, Ken A. Cornell, Jared J. Romero, et al.) International Journal of Molecular Sciences (2020)

The Center of Biomedical Research Excellence in Matrix Biology strives to improve our understanding of extracellular matrix at molecular, cellular, tissue, and organismal levels to generate new knowledge about pathophysiology, normal development, and regenerative medicine. ...

Combating Osteoporosis and Obesity with Exercise: Leveraging Cell Mechanosensitivity (Gabriel M. Pagnotti, Maya Styner, Gunes Uzer, et al.) Nature Reviews: Endocrinology (2019)

Osteoporosis, a condition of skeletal decline that undermines quality of life, is treated with pharmacological interventions that are associated with poor adherence and adverse effects. Complicating efforts to improve clinical outcomes, the incidence of obesity ...

Recovery of Stem Cell Proliferation by Low Intensity Vibration Under Simulated Microgravity Requires LINC Complex (H. Touchstone, R. Bryd, S. Loisate, et al.) Microgravity (2019)

Mesenchymal stem cells (MSC) rely on their ability to integrate physical and spatial signals at load bearing sites to replace and renew musculoskeletal tissues. Designed to mimic unloading experienced during spaceflight, preclinical unloading and simulated ...

Sun-Mediated Mechanical LINC Between Nucleus and Cytoskeleton Regulates βcatenin Nuclear Access (Gunes Uzer, Guniz Bas and Scott Birks) Journal of Biomechanics (2018)

βcatenin acts as a primary intracellular signal transducer for mechanical and Wnt signaling pathways to control cell function and fate. Regulation of βcatenin in the cytoplasm has been well studied but βcatenin nuclear trafficking and ...

Enucleated Cells Reveal Differential Roles of the Nucleus in Cell Migration, Polarity, and Mechanotransduction (Gunes Uzer) Journal of Cell Biology (2018)

The nucleus has long been postulated to play a critical physical role during cell polarization and migration, but that role has not been defined or rigorously tested. Here, we enucleated cells to test the physical ...

LARG GEF and ARHGAP18 Orchestrate RhoA Activity to Control Mesenchymal Stem Cell Lineage (Gunes Uzer) Bone (2018)

The quantity and quality of bone depends on osteoblastic differentiation of mesenchymal stem cells (MSCs), where adipogenic commitment depletes the available pool for osteogenesis. Cell architecture influences lineage decisions, where interfering with cytoskeletal structure promotes ...

Intranuclear Actin Regulates Osteogenesis (Buer Sen, Zhihui Xie, Gunes Uzer, et al.) Stem Cells (2015)

Depolymerization of the actin cytoskeleton induces nuclear trafficking of regulatory proteins and global effects on gene transcription. We here show that in mesenchymal stem cells (MSCs), cytochalasin D treatment causes rapid cofilin-/importin-9-dependent transfer of G-actin ...

Cell Mechanosensitivity to Extremely Low-Magnitude Signals Is Enabled by a LINCed Nucleus (Gunes Uzer) Stem Cells (2015)

A cell's ability to recognize and adapt to the physical environment is central to its survival and function, but how mechanical cues are perceived and transduced into intracellular signals remains unclear. In mesenchymal stem cells ...

Osteocyte Specific Responses to Soluble and Mechanical Stimuli in a Stem Cell Derived Culture Model (Gunes Uzer) Scientific Reports (2015)

Studying osteocyte behavior in culture has proven difficult because these embedded cells require spatially coordinated interactions with the matrix and surrounding cells to achieve the osteocyte phenotype. Using an easily attainable source of bone marrow ...

Nuclear Envelope Mechanosome Regulates βcatenin Nuclear Transport (Gunes Uzer, G. Bas, W. R. Thompson, et al.) ORS 45th Sun Valley Workshop, Musculoskeletal Biology (2015)

Wnt/LRP/β-Catenin Signaling in Bone (Gunes Uzer) University of North Carolina, School of Medicine, Division of Endocrinology Grant Rounds (2013)

Role of Fluid Shear Modulation on Bone Cell Metabolism During High-Frequency Oscillatory Vibrations (Gunes Uzer) Stony Brook University, Department of Biomedical Engineering (2012)

Mechanical Environment During High-Frequency Vibrations In-Vitro (Gunes Uzer) Department of Biomedical Engineering, Stony Brook University (2011)

Mechanotransduction in Stem Cells (Alejandra Almaraz, Paul Davis, Sean Howard, et al.) 2023 Undergraduate Research Showcase (2023)

Scientists have discovered that prolonged exposure to microgravity leads to degenerative changes in both bone and muscle tissue, but the precise mechanism behind this remains unknown. It is known, however, that the LINC complex pathway ...

Probing the Nanomechanical Behavior of Cells and Cell Nuclei (Jesse Schimpf, Josh Newberg, Gunes Uzer, et al.) 2019 Undergraduate Research and Scholarship Conference (2019)

Atomic force microscopy (AFM) is a nanoscale characterization technique that at its most basic level employs a nanometer-scale probe tip to physically trace a surface, generating a topographical map of the sample. However AFM has ...