Skip to main content
Cationic Nanoparticles Induce Nanoscale Disruption in Living Cell Plasma Membranes
Journal of Physical Chemistry B (2009)
  • Jiumei Jiumei
  • Jessica A. Hessler
  • Krishna Putchakayala
  • Brian K. Panama
  • Damian P. Khan
  • Seungpyo Hong
  • Douglas G. Mullen
  • Stassi C. DiMaggio, Xavier University
  • Abhigyan Som, University of Massachusetts - Amherst
  • Gregory N. Tew, University of Massachusetts - Amherst
  • Anatoli N. Lopatin
  • James R. Baker Jr.
  • Mark M. Banaszak Holl
  • Bradford G. Orr

It has long been recognized that cationic nanoparticles induce cell membrane permeability. Recently, it has been found that cationic nanoparticles induce the formation and/or growth of nanoscale holes in supported lipid bilayers. In this paper, we show that noncytotoxic concentrations of cationic nanoparticles induce 30−2000 pA currents in 293A (human embryonic kidney) and KB (human epidermoid carcinoma) cells, consistent with a nanoscale defect such as a single hole or group of holes in the cell membrane ranging from 1 to 350 nm2 in total area. Other forms of nanoscale defects, including the nanoparticle porating agents adsorbing onto or intercalating into the lipid bilayer, are also consistent; although the size of the defect must increase to account for any reduction in ion conduction, as compared to a water channel. An individual defect forming event takes 1−100 ms, while membrane resealing may occur over tens of seconds. Patch-clamp data provide direct evidence for the formation of nanoscale defects in living cell membranes. The cationic polymer data are compared and contrasted with patch-clamp data obtained for an amphiphilic phenylene ethynylene antimicrobial oligomer (AMO-3), a small molecule that is proposed to make well-defined 3.4 nm holes in lipid bilayers. Here, we observe data that are consistent with AMO-3 making ∼3 nm holes in living cell membranes.

Publication Date
Publisher Statement
The published version is located at
Citation Information
Jiumei Jiumei, Jessica A. Hessler, Krishna Putchakayala, Brian K. Panama, et al.. "Cationic Nanoparticles Induce Nanoscale Disruption in Living Cell Plasma Membranes" Journal of Physical Chemistry B Vol. 113 Iss. 32 (2009)
Available at: