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Article
Heterodera glycines Cyst Components and Surface Disinfestants Affect H. glycines Hatching
Journal of Nematology
  • D. V. Charlson, Iowa State University
  • G. L. Tylka, Iowa State University
Document Type
Article
Publication Version
Published Version
Publication Date
12-1-2003
Abstract
We investigated the effects of Heterodera glycines cyst components and surface disinfestants on hatching of H. glycines eggs in vitro. Eggs were incubated in either H. glycines cyst wall fragments, cyst wall and egg rinsate, egg homogenate, or control solutions of soybean root diffusate, sterile distilled water, or zinc sulfate. Hatch in cyst wall and egg rinsate, and egg homogenate, was greater ([alpha] = 0.05) than hatch in sterile distilled water; however, it was not different from hatch in zinc sulfate according to Dunnett's test. Hatch in cyst wall fragments was similar to hatch in sterile distilled water. To determine whether surface disinfestants affected hatch, eggs were treated first with chlorhexidine diacetate, mercuric chloride, sodium hypochlorite, or streptomycin sulfate and then incubated in H. glycines egg homogenate, soybean root diffusate, sterile distilled water, or zinc sulfate. Hatch of eggs treated with chlorhexidine diacetate, mercuric chloride, and streptomycin sulfate was reduced ([alpha] = 0.05), and hatch of eggs treated with sodium hypochlorite was increased ([alpha] = 0.05) relative to hatch of nontreated eggs in all incubation solutions except zinc sulfate according to Dunnett's Test. Hatch in zinc sulfate was similar among all surface disinfestants except mercuric chloride, where hatch was reduced relative to hatch of nontreated and other surface disinfestant-treated eggs.
Comments

This article is published as Charlson, D. V., and G. L. Tylka. "Heterodera glycines Cyst Components and Surface Disinfestants Affect H. glycines Hatching." Journal of Nematology 35, no. 4: 458-464. Posted with permission.

Copyright Owner
The Society of Nematologists
Language
en
File Format
application/pdf
Citation Information
D. V. Charlson and G. L. Tylka. "Heterodera glycines Cyst Components and Surface Disinfestants Affect H. glycines Hatching" Journal of Nematology Vol. 35 Iss. 4 (2003) p. 458 - 464
Available at: http://works.bepress.com/gregory-tylka/199/