In May 2017 I became (an) image core manger at Johns Hopkins University School of Medicine in Baltimore, Md. http://confocal.jhu.edu/gallery and still continue as a part time biomedical consultant in T-cell immunotherapy (congratulations to Novartis and Gilead/Kite on FDA approvals of CAR T-cells and congratulations to Gilead and Kite on acquisition).

In April 2016 my MDACC funding ran out so I am became an independent biomedical consultant.I currently post more frequently at

https://www.linkedin.com/in/georgemcnamara

and have some content at

than here at bepress/selectedworks.-- in part because bepress has not so far added blogging (nor have they implemented my suggestion to link users here with our linkedin profile and content).

My linkedin Pulse blogs often have multiple parts to them, so just skimming the title (and ultra cool headline photos) do not tell the whole story. For example,

discusses my critique of the June 2016 NIH RAC meeting (UPenn transducing CD19 CAR into CD19 B-cell actute lymphocytic leukemia resulted in a CD19- B-ALL relapse ... I suggest how to avoid tis in the future), followed by my multi-CAR proposal to cure cancer, with specific list of oncosurfaceome targets for the deadly stage 4 glioblastoma,

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In April 2013 I became Single Cell Analyst (Senior Research Scientist) in the laboratory of Prof. Laurence J.N. Cooper, University of Texas M.D. Anderson Cancer Center, Houston, TX. One reason for moving to LC's lab is that I invented a way to multiplex many fluorescent protein reporters in live cells. This "Tattetales" concept is explained in documents inside the zip file where at

See also

http://works.bepress.com/gmcnamara/75.

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I want to thank my former colleagues at the University of Miami for the almost 6 years I spent there.

While I was mag core manger at the University of Miami, Miller School of Medicine, we submitted two custom Synergy STED nanoscopes (9/2011, one to NIH, other a Leica confocal upgrade to DoD DURIP). Our expectation is that Synergy STED will take us to 50x50x200 nm XYZ resolution with 4 fluorescence colors (see http://archiv.ub.uni-heidelberg.de/volltextserver/volltexte/2011/11539/pdf/Reuss_Dissertation.pdf for initial one color 3D-EasySTED and Reuss … Hell 2010 Optics Express for 2D-EasySTED and MOM-STED). This resolution is much better than confocal microscopy, which is limited to 214x214x600 nm (1.4 NA objective lens, 500 nm) and to the current Leica CW-STED (80x80x~600 nm, any color you want as long as it is green). We also believe that Synergy STED’s resolution will enable precision localization (a’la PALm, STORM, FPALM) of single molecules to 20x20nm XY resolution using conventional fluorescent dye labeled antibodies (that is, stable fluorescent dyes, not relying on most fluorophores being off).

We named this custom nanoscope Synergy STED because we take advantage of the synergy between 3D-EasySTED segmented waveplate(s) (www.b-halle.de), the new Coherent Chameleon Ultra II multiphoton laser + Chameleon Compact OPO-Vis (together, 370 to 1600 nm wavelength range with 80 MHz >100 mW pulses), Leica SP5-II confocal microscope, PicoQuant TCSPC FLIM for time-gated g-STED, and ultrastable TMC vibration isolation table. In particular, we learned from the Goetz Zinner of B-Halle, that each 3D-EasySTED SWP could be used with multiple excitation + STED depletion laser lines (see the Excel file we posted here).

In 11/2011 (post submission) we learned of www.APE-Berlin/de HarmoniXX SHG+THG and are curious whether Chameleon laser + OPO (680-1300 nm) + HarmoniXX (227-650 nm) would meet our needs even better (one of our users would like 300 nm pulses). Perhaps it will even be possible (both price and technical feasibility) to put two HarmoniXX SHG+THG units on the instrument, one on the Chameleon laser 680-1040nm output for SHG (340-520nm) and THG (227 to 346 nm) and on the OPO 1,000-1,600nm output for SHG (500-800nm) and THG (333-533nm). If you want to see this all work, feel free to write a check to enable us to buy and integrate this equipment.