Fluorescence microscopy data acquired for 3B Bayesian localization microscopy.
Settings: 100 nm XY pixel size. 50 millisecond exposures, MetaMorph stream acquisition mode (as fast as possible). Stellaris FISH mammalian cells labeled with Quasar 670 fluorophores, one molecule per 20mer FISH probe, ~48 probes to TOP1 mRNA (Topoisomerase 1).
More on the 3B project and Stellaris FISH data at http://works.bepress.com/gmcnamara/38/
and at Susan Cox's web sites http://www.coxphysics.com/3b/ http://superresolved.com/
- Bayesian localization
Available at: http://works.bepress.com/gmcnamara/40/