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Time series data for 3B image processing
  • George McNamara, M.D. Anderson Cancer Center

Time series data for 3B image processing

Four time series data sets of Stellaris single molecules RNA FISH (fluorescence in situ hybridization). All four datasets are 301 imaqe planes. FISH probes are to TOP1 mRNA (Topoisomerase I)in Saos osterosarcoma cells.

One dataset is 10 millisecond exposures (very dim), acquired in Streaming acquisition mode (no hardware overhead).

The other three datasets are a three consecutive planes of the same XY field of view.

I have included image processing results for:

PiMP - a fast method developed by Sebastian Munck et al 2012,

and greatly improved performance by Glen Macdonald (Seattle).

3B microscopy - Susan Cox and Ed Rosten

3B = Bayesian analysis of blinking and bleaching.

The 3B result is of a small field of view with date/time "stamps" as part of the filenames.

  • Time series,
  • image processing,
  • super-resolution,
  • nanoscopy,
  • 3B,
  • PiMP
Publication Date
Winter January 22, 2014
Data are facts. Facts are not subject to copyright (at least in the USA).
Citation Information
George McNamara. "Time series data for 3B image processing" (2014)
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