McNamara 20131101Fri Leica widefield microscope CUDA Deconvolution Stellaris FISH probe cultured cells dataset #1.zip
A text file in the zip archive has experiment details. I am posting this online so that researchers - whether academic or commercial - can evaluate the acquired data, the Bruce and Butte 2013 Optics Express ( http://www.opticsinfobase.org/oe/fulltext.cfm?uri=oe-21-4-4766 ) deconvolution result (note: I may not have used optimal settings), and to compare these deconvolution results to other methods. If anyone generates alternative spatial deconvolution output, such as from: * SVI Huygens * Media Cy AutoQuant * Agard's ER-Decon (Arigovindan 2013 PNAS) * Vicidomini SGP GPU Deconv (Zanella 2013 Sci Rep) or other, please post the data online and let me know where to find it.
I am very interested in spectral unmixing. Especially: * autofluorescence (GFPcube channel) vs fluorescent dyes. * Quasar 570 vs Cal Fluor Red 610 (nominally Cy3cube vs TxRed cube). I plan to upgrade the microscope to get better optical separation of fluorophores, but have clear need for "spectral unmixing" and/or other methods of fluorophore channels separation.
My thanks to Sally and Marc at Biosearch Technologies for the slide.
- single molecules FISH,
- mRNA FISH,
- fluorescence in situ hybridization,
- CUDA GPU deconvolution,
- oil immersion objective lens,
- Stellaris FISH,
- widefield microscope,
- 5plex fluorescence
Available at: http://works.bepress.com/gmcnamara/31/