Hamamatsu FLASH4.0 scientific cMOS camera exposure time series are pairs of images of:
1 millisecond (00,001ms series)
10 millisecond (00,010ms series)
100 millisecond (00,100ms series)
1,000 millisecond (01,000ms series)
4,000 millisecond (04,000ms series)
10,000 millisecond (10,000ms series)
I also included:
* difference images (exposure 2 minus exposure 1 plus 100 intensity values).
* a series of eleven 1 second (1,000 ms) exposure time images in a multi-plane TIFF file (different images than the pair of 1,000ms images above).
* Stack Arithmetic: Median, Average, Minimum, Maximum, of the eleven plane series (Stack Arithmetic is a MetaMorph command).
These images were acquired with MetaMorph Imaging System 7.8 on a Leica DMI 6000 inverted microscope with no illumination. A box covered the eyepiece port (necessary because otherwise light leaks in through the eyepieces and reaches the camera, regardless of which direction the eyepieces-left camera port-right camera port is selected).
- dark reference,
- pixel gain
Available at: http://works.bepress.com/gmcnamara/29/