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Comprehensive Off-Line, Two-Dimensional Liquid Chromatography. Application to the Separation of Peptide Digests
Analytical Chemistry (2008)
  • Georges Guiochon, University of Tennessee - Knoxville
  • Nicola Marchetti, University of Tennessee - Knoxville
  • Jacob N. Fairchild, University of Tennessee - Knoxville
Abstract
The separation of the peptide digests of myoglobin and bovine serum albumin was performed with an off-line combination of two commercial, conventional HPLC columns. The first column was packed with a strong ion exchanger and eluted with a KCl gradient. The second column was packed with particles of C18-bonded silica and eluted with an acetonitrile gradient. The conditional peak capacities of the 2D separations achieved exceed 7000 under the experimental conditions investigated. This performance is achieved at the cost of an analysis time of the order of 28 hours. Possible improvements to the separation method described here are discussed.
Publication Date
March, 2008
Citation Information
Georges Guiochon, Nicola Marchetti and Jacob N. Fairchild. "Comprehensive Off-Line, Two-Dimensional Liquid Chromatography. Application to the Separation of Peptide Digests" Analytical Chemistry Vol. 80 Iss. 8 (2008)
Available at: http://works.bepress.com/georges_guiochon/14/