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Article
Identification of light-independent inhibition of human immunodeficiency virus-1 infection through bioguided fractionation of Hypericum perforatum
Virology Journal
  • Wendy Maury, University of Iowa
  • Jason P. Price, University of I owa
  • Melinda A. Brindley, University of Iowa
  • ChoonSeok Oh, University of Iowa
  • Jeffrey D. Neighbors, University of Iowa
  • David F. Wiemer, University of Iowa
  • Nickolas Wills, Iowa State University
  • Susan L. Carpenter, Iowa State University
  • Catherine C. Hauck, Iowa State University
  • Patricia A. Murphy, Iowa State University
  • Mark P. Widrlechner, United States Department of Agriculture
  • Kathleen Delate, Iowa State University
  • Ganesh Kumar, Iowa State University
  • George A. Kraus, Iowa State University
  • Ludmila Rizshsky, Iowa State University
  • Basil J. Nikolau, Iowa State University
Document Type
Article
Publication Version
Published Version
Publication Date
7-13-2009
DOI
10.1186/1743-422X-6-101
Abstract
BackgroundLight-dependent activities against enveloped viruses in St. John's Wort (Hypericum perforatum) extracts have been extensively studied. In contrast, light-independent antiviral activity from this species has not been investigated. Results Here, we identify the light-independent inhibition of human immunodeficiency virus-1 (HIV-1) by highly purified fractions of chloroform extracts of H. perforatum. Both cytotoxicity and antiviral activity were evident in initial chloroform extracts, but bioassay-guided fractionation produced fractions that inhibited HIV-1 with little to no cytotoxicity. Separation of these two biological activities has not been reported for constituents responsible for the light-dependent antiviral activities. Antiviral activity was associated with more polar subfractions. GC/MS analysis of the two most active subfractions identified 3-hydroxy lauric acid as predominant in one fraction and 3-hydroxy myristic acid as predominant in the other. Synthetic 3-hydroxy lauric acid inhibited HIV infectivity without cytotoxicity, suggesting that this modified fatty acid is likely responsible for observed antiviral activity present in that fraction. As production of 3-hydroxy fatty acids by plants remains controversial, H. perforatum seedlings were grown sterilely and evaluated for presence of 3-hydroxy fatty acids by GC/MS. Small quantities of some 3-hydroxy fatty acids were detected in sterile plants, whereas different 3-hydroxy fatty acids were detected in our chloroform extracts or field-grown material. ConclusionThrough bioguided fractionation, we have identified that 3-hydroxy lauric acid found in field grown Hypericum perforatum has anti-HIV activity. This novel anti-HIV activity can be potentially developed into inexpensive therapies, expanding the current arsenal of anti-retroviral agents.
Comments

This article is from Virology Journal 6 (2009): 101, doi:10.1186/1743-422X-6-101. Posted with permission.

Rights
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Copyright Owner
Maury et al
Language
en
Date Available
February 10, 2013
File Format
application/pdf
Citation Information
Wendy Maury, Jason P. Price, Melinda A. Brindley, ChoonSeok Oh, et al.. "Identification of light-independent inhibition of human immunodeficiency virus-1 infection through bioguided fractionation of Hypericum perforatum" Virology Journal Vol. 6 (2009) p. 101
Available at: http://works.bepress.com/george_kraus/3/