|2004 - Present||Affiliated Faculty, Nova Southeastern University ‐ Halmos College of Natural Sciences and Oceanography|
|1972 - 2017||Criminalist I, II, III; DNA Unit Manager, Broward Sheriff's Office ‐ DNA Unit|
|1996 - 2010||Courtesy Professor Appointment; Adjunct Professor, Florida International University ‐ Chemistry Department|
|1985 - 2003||Adjunct Chemistry Instructor, Broward Community College|
|1970 - 1972||Lab Technician, Crime Scene Unit, Police Officer, Dade County Public Safety Department - Crime Laboratory|
|1969 - 1970||Criminalist Intern, Dade County Crime Laboratory|
Professional Service and Affiliations
|1992 - Present||Member, American Association for the Advancement of Science||1974 - Present||Member, American Academy of Forensic Sciences, Emeritus||1970 - Present||Member, Past President (South Florida Subsection), American Chemical Society (ACS), Emeritus||1994 - 2010||Member, American Society of Human Genetics||1991 - 2008||Member, Forensic Science Society (Great Britain)||2004||Member, Association of Forensic DNA Examiners and Administrators (AAFDA)||1989 - 2001||Member, Technical Working Group on DNA Analysis Methods (TWGDAM)|
Honors and Awards
- American Board of Criminalistics Emeritus, Diplomate, Molecular Biology, 2015 - present
- American Board of Criminalistics, Fellow, Molecular Biology, 2005 - 2015
- American Board of Criminalistics, Diplomate, General Criminalistics, since 1997
- Licensed Technologist in Clinical Chemistry and Immunohematology (Department of Rehabilitative Services, since 1971 [presently inactive status])
|1996||Ph.D. -- Biology, Florida International University|
|1983||M.S. - Chemistry, Florida Atlantic University|
|1970||B.S. - ACS Chemistry, University of Miami|
Development of a Microfluidic Device (μPADs) for Forensic Serological Analysis Analytical Methods (2019)
In this paper, we describe a paper microfluidic device capable of performing a variety of presumptive tests for the presence of biological fluids at crime scenes. The device is multiplexed and permits the simultaneous detection ...
A Novel On‐Chip Method for Differential Extraction of Sperm in Forensic Cases Advanced Science (2018)
One out of every six American women has been the victim of a sexual assault in their lifetime. However, the DNA casework backlog continues to increase outpacing the nation's capacity since DNA evidence processing in ...
Internal validation of STRmix™ – A multi laboratory response to PCAST Forensic Science International: Genetics (2018)
We report a large compilation of the internal validations of the probabilistic genotyping software STRmix™. Thirty one laboratories contributed data resulting in 2825 mixtures comprising three to six donors and a wide range of multiplex, ...
FlipTube™ technology promotes clean manipulation of forensic samples on automated robotic workstations Forensic Science International: Genetics Supplement Series (2017)
One method to reduce forensic DNA crime laboratory casework backlogs is to rethink DNA sample preparation and analysis processing; often using automated liquid handling platforms that are both robust and performance-verified. This enables forensic scientists ...
Developmental validation studies of epigenetic DNA methylation markers for the detection of blood, semen and saliva samples Forensic Science International: Genetics (2016)
Determining the type and origin of body fluids in a forensic investigation can provide important assistance in reconstructing crime scenes. A set of epigenetic markers, ZC3H12D, BCAS4 and cg06379435, have been developed to produce unique ...
Forensic discrimination of vaginal epithelia by DNA methylation analysis through pyrosequencing Electrophoresis (2016)
The accurate identification of body fluids from crime scenes can aid in the discrimination between criminal and innocent intent. This research aimed to determine if the levels of DNA methylation in the locus PFN3A could ...
High-resolution melt analysis of DNA methylation to discriminate semen in biological stains Analytical Biochemistry (2016)
The goal of this study was to develop a method for the detection of semen in biological stains using high-resolution melt (HRM) analysis and DNA methylation. To perform this task, we used an epigenetic locus ...
Evaluation of DNA methylation markers and their potential to predict human aging Electrophoresis (2015)
We present epigenetic methylation data for two genetic loci, GRIA2, and NPTX2, which were tested for prediction of age from different donors of bioﬂuids. We analyzed 44 saliva samples and 23 blood samples from volunteers ...
An Investigation of PCR Inhibition Using Plexor®-Based Quantitative PCR and Short Tandem Repeat Amplification Journal of Forensic Sciences (2014)
A common problem in forensic DNA typing is PCR inhibition resulting in allele dropout and peak imbalance. In this paper, we have utilized the Plexor® real-time PCR quantification kit to evaluate PCR inhibition. This is ...
Identification of spermatozoa by tissue-specific differential DNA methylation using bisulfite modification and pyrosequencing Electrophoresis (2014)
The focus of this study is to evaluate the application of epigenetic markers as a forensic tool for the determination of semen present in sexual assault cases. A series of genetic loci were screened in ...
High-resolution melt analysis of the minisatellite D1S80: A potential forensic screening tool Electrophoresis (2014)
High-resolution melt (HRM) analysis of the VNTR region of the human D1S80 locus, a 16-bp repeat minisatellite from approximately 400 to over 700 bp in length, was investigated. A Qiagen Rotor-Gene Q using the Type-it ...
The determination of tissue-specific DNA methylation patterns in forensic biofluids using bisulfite modification and pyrosequencing Electrophoresis (2012)
The goal of this study is to explore the application of epigenetic markers in the identification of biofluids that are commonly found at the crime scene. A series of genetic loci were examined in order ...
Y chromosome STR allelic and haplotype diversity in a Rwanda population from East Central Africa Legal Medicine (2012)
We have analyzed 17 Y-chromosomal STR loci in a population sample of 69 unrelated male individuals of the Rwanda-Hutu population from East Central Africa using an AmpF l STR® Yfiler™ PCR amplification kit. A total ...
Mutation at the Human D1S80 Minisatellite Locus The Scientific World Journal (2012)
Little is known about the general biology of minisatellites. The purpose of this study is to examine repeat mutations from the D1S80 minisatellite locus by sequence analysis to elucidate the mutational process at this locus. ...
Genetic variation of 15 autosomal microsatellite loci in a Tamil population from Tamil Nadu, Southern India Legal Medicine (2010)
The genetic profiles for 15 autosomal microsatellite loci were analyzed in a Tamil population from Southern India to study the genetic diversities and relatedness of this population with other national and global populations. Statistical analyses ...
Investigating SNPs Flanking the D1S80 Locus in a Tamil Population from India Human Biology (2010)
D1S80 is a 16-bp variable number of tandem repeats minisatellite. We analyzed single nucleotide polymorphisms (SNPs) flanking this locus in a Tamil population. Alleles ranged from 15 through 41 repeats, with alleles 18 and 24 ...
A Low-Cost, High-Throughput, Automated Single Nucleotide Polymorphism Assay for Forensic Human DNA Applications Analytical Biochemistry (2009)
Single nucleotide polymorphism (SNP) analysis of human DNA for the purpose of identification has some promising attributes. The question of approach is critical to the eventual adoption of this technology. The use of a low-volume ...
D1S80 Single-Locus Discrimination Among African Populations Human Biology (2004)
The highly polymorphic D1S80 locus has no known genetic function. However, this variable number of tandem repeats (VNTR) locus has been highly valuable in forensic identification. In this study we report the allele and genotype ...
Distribution of the HLA-DQA1 and Polymarker Alleles in the Basque Population of Spain Forensic Science International (2000)
HLA-DQA1 and polymarker (LDLR, GYPA, HBGG, D7S8, and GC) genotypic and allelic frequencies are determined for a population sample of 102 unrelated Basque individuals using PCR-based methodology. All six loci met Hardy–Weinberg expectations in at ...
Microvariation at the Human D1S80 Locus International Journal of Legal Medicine (1997)
The minisatellite locus D1S80, (location: 1p35– p36), GenBank sequence accession # D28507), is a variable number of tandem repeat (VNTR) locus with a 16 base pair repeat size. The sequence of the predominant core repeat ...
Development of a Deoxyribonucleic Acid (DNA) Restriction Fragment Length Polymorphism (RFLP) Database for Punjabis in East Punjab, India Forensic Science International (1996)
In response to continuing interest in obtaining reference deoxyribonucleic acid (DNA) analysis data for previously unstudied population groups, blood samples were collected from Punjabi individuals living in East Punjab, India. This first segment of our ...
Polymorphic Human Specific Alu Insertions as Markers for Human Identification Electrophoresis (1995)
Alu sequences represent the largest family of short interspersed repetitive elements (SINEs) in humans with 500 000 copies per genome. Recently, one Alu subfamily was found to be human specific (HS). We originally described the ...